Mouse Spleen Dissociation
Mouse Spleen Dissociation - Related Videos
05:18
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
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Cited by 22 •
2008
This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.
04:47
A Mouse Model of Vascularized Heterotopic Spleen Transplantation
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2025
The video demonstrates a surgical method for generating a mouse model of vascularized heterotopic spleen transplantation. The abdominal cavity of an anesthetized donor mouse is surgically opened to remove the spleen. The inferior vena cava and infrarenal aorta are identified, their lumbar branches are ligated, and openings are made for the heterotopic transplantation of a spleen from a congenic donor mouse. Upon completing vascular anastomosis and restoring blood flow to the implanted spleen,...
02:03
Assessing Bacterial Burden in the Mouse Spleen for Infection Studies
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2026
Source: Madenspacher, J. H. and Fessler, M. B. A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice. J. Vis. Exp. (2016)This video demonstrates the process of quantifying bacterial load in the spleen of an infected mouse to assess the severity of infection. The homogenized spleen is serially diluted and plated to determine total bacterial burden. The resulting data provide insights into host-pathogen interactions within...
03:52
Quantification of Bacterial Load in Mouse Spleen and Liver After Systemic Infection
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2026
Source: Ahn, J. J., et al. Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses. J. Vis. Exp. (2016)The video demonstrates bacterial load measurement in spleen and liver tissues isolated from mice infected with an intracellular bacterial pathogen. Bacteria, accumulated within tissue-resident phagocytes during systemic infection, are released using detergent-based lysis and mechanical disruption. Serial dilutions of the homogenates are plated on...
18:02
Preparation of Dissociated Mouse Cortical Neuron Cultures
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Cited by 146 •
2007
This video shows a procedure for generating neuronal cultures from late embryo and early postnatal mouse cortex. These cultures can be used for immunocytochemistry, biochemistry, electrophysiology, calcium and sodium imaging and provide a platform to study the neuronal development of transgenic animals that carry a postnatal lethal gene mutation.
FAQs
Mechanical disruption breaks the splenic tissue into smaller pieces, while gentle trituration helps release individual cells from the disrupted material. These steps must balance effective cell release with protection of cell viability and surface markers. The resulting quality directly affects whether immune populations can be measured consistently in downstream flow cytometry, culture, microscopy, or molecular analyses.
Filtration removes larger tissue fragments and debris from the cell suspension, producing a cleaner preparation for analysis. Red blood cell lysis can be added when erythrocytes interfere with sample quality or the intended assay. Because these steps address different components of the preparation, filtration and lysis serve complementary purposes rather than interchangeable ones.
Dissociation conditions influence both cell viability and the preservation of surface markers used to identify populations. Excessive disruption or inconsistent handling can therefore reduce the reliability of comparisons between samples, even when the same tissue source is examined. Standardized conditions support more reproducible measurements of lymphocytes, macrophages, and other splenic populations.
A single-cell preparation makes different splenic populations accessible to separate analytical methods. Researchers can examine lymphocytes, macrophages, and other cells through flow cytometry, microscopy, culture, or molecular analysis, depending on the study design. This helps connect cellular composition with broader questions about immune-system function, infection, cancer, and other biological conditions.
The workflow begins with mechanical disruption of splenic tissue, followed by gentle trituration to release cells. The suspension is then passed through a filter to remove debris. If required by the sample or downstream analysis, red blood cell lysis is included to improve preparation quality. The resulting suspension is directed to the selected analytical application.
The resulting suspension can be adapted for flow cytometry, cell culture, microscopy, or molecular analyses. Flow cytometry can examine splenic cell populations, while microscopy and culture provide other ways to study cells after preparation. Molecular analyses can address tissue or cellular characteristics. The appropriate endpoint depends on which populations and biological features the investigation targets.
Mouse spleen dissociation is useful when investigators need to compare splenic immune-cell populations or examine tissue composition at the cellular level. It supports research on immunology, infection, cancer, and immune-system function. Maintaining comparable dissociation conditions is especially important in these studies because sample preparation can influence viability, marker preservation, and the reliability of measured differences.