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Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
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Biology
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Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

DOI:
10.3791/1029-v

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05:18 min

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December 11, 2008

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, , , ,
1Miltenyi Biotec,GmbH

Chapters

  • 00:00Title
  • 00:26Introduction
  • 00:58Introduction to Equipment
  • 01:55Dissociating the spleen using the gentleMACS Dissociator
  • 03:42Filtration of dissociated spleen
  • 04:49Conclusion

Summary

Automatic Translation

Automatic Translation

This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.

Tags

Single-Cell SuspensionsMouse SpleenGentleMACS DissociatorExperimentsCell SeparationCell AnalysisCell CultureManual DissociationControlled ConditionsReproducible ConditionsUser-safetyVideo TutorialAutomated DeviceViable Cell SuspensionsFilteringCentrifugationResuspension

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