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Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
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Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Article DOI: 10.3791/1029 • 05:18 min • December 11th, 2008
December 11th, 2008 •
1, 1, 1, 1, 1
1Miltenyi Biotec,GmbH

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This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.

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Single-Cell Suspensions Mouse Spleen GentleMACS Dissociator Experiments Cell Separation Cell Analysis Cell Culture Manual Dissociation Tissue Dissociation Controlled Conditions Reproducible Conditions User-safety Video Tutorial Automated Device Viable Cell Suspensions Filtering Centrifugation Resuspension
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