Muscle Dissociation
Muscle Dissociation - Related Videos
08:16
Utility of Dissociated Intrinsic Hand Muscle Atrophy in the Diagnosis of Amyotrophic Lateral Sclerosis
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Cited by 12 •
2014
Dissociated atrophy of intrinsic hand muscles, termed the split hand, appears to be a specific feature of amyotrophic lateral sclerosis (ALS). Consequently, a novel neurodiagnostic test, termed the split hand index, was developed to quantify the clinical phenomenon of the split hand, which differentiated ALS from mimic disorders.
08:12
Intact Short, Intermediate, and Long Skeletal Muscle Fibers Obtained by Enzymatic Dissociation of Six Hindlimb Muscles of Mice: Beyond Flexor Digitorum Brevis
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Cited by 2 •
2023
We describe a protocol to obtain enzymatically dissociated fibers of different lengths and types from six muscles of adult mice: three of them already described (flexor digitorum brevis, extensor digitorum longus, soleus) and three of them successfully dissociated for the first time (extensor hallucis longus, peroneus longus, peroneus digiti quarti).
05:58
Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations
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Cited by 21 •
2013
Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.
01:27
Dissociative Disorders
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2025
Dissociative disorders represent complex psychological conditions characterized by disruptions in consciousness, memory, identity, or perception. These disruptions cause individuals to experience a disconnection from their thoughts, emotions, and memories. The phenomenon is not merely an occasional lapse in attention but a profound alteration in mental functioning that can severely impact daily life. Dissociative Fugue A hallmark feature of dissociative disorders is the dissociative fugue...
01:21
Dissociative Amnesia
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2025
Dissociative amnesia is a complex psychological condition that manifests as an inability to recall personal information, often tied to traumatic or stressful events. Unlike general amnesia, individuals with this condition retain the ability to perform routine activities and procedural tasks, such as operating a phone or navigating public transportation, yet experience profound gaps in autobiographical memory. These lapses may encompass significant life events, such as suicide attempts or...
FAQs
Enzymatic digestion and mechanical trituration perform complementary tasks. Collagenase or related proteases break down the extracellular matrix that holds muscle cells within tissue, while gentle trituration helps separate the loosened material into individual cells or small populations. Using both steps supports cellular recovery while reducing handling effects that can compromise viability or alter cell-surface markers.
These conditions influence how effectively tissue is loosened and how well cells remain suitable for downstream analysis. Poor control can disrupt the balance between enzymatic digestion and preservation of viability or surface markers. Careful adjustment is particularly important when the resulting suspension will be used for culture, flow cytometry, transplantation, or cellular studies of muscle regeneration.
Separated cells allow researchers to examine muscle at a finer scale than an intact tissue sample provides. This cellular resolution helps connect tissue-level structure with cellular and molecular measurements. It is especially valuable when distinct populations, including satellite cells, must be studied in relation to muscle regeneration, development, or disease rather than treated as part of one uniform tissue.
A typical workflow begins by exposing intact muscle tissue to collagenase or a related protease so the extracellular matrix is digested. The loosened material then undergoes gentle mechanical trituration, with temperature, incubation time, and handling controlled throughout. The resulting suspension can support primary culture, cell isolation, flow-cytometry analysis, transplantation studies, or other cellular investigations.
The method is useful when investigators need primary muscle-cell culture, satellite-cell isolation, flow-cytometry measurements, or transplantation studies. It also supports research on muscle regeneration, development, and disease by making cellular populations accessible after tissue separation. These applications allow experiments to address questions that cannot be resolved as directly from intact muscle tissue alone.
A dissociated sample provides access to individual cells or small cell populations for cellular and molecular measurements. Researchers can assess material in a format compatible with downstream analyses while considering whether viability and surface markers were preserved during preparation. In biology, this creates a practical link between organized muscle structure and investigations focused on specific cellular populations or processes.