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Biology

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Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations
 

Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations

Article doi: 10.3791/50259
November 13th, 2013

Summary November 13th, 2013

Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.

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