Plant Transformation
Plant Transformation - Related Videos
05:09
Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants: A Technique to Generate Transgenic Plants With Single-Copy Insertion
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2025
In this video, we demonstrate generation of transgenic Arabidopsis plants expressing stable transgene through Agrobacterium-mediated binary bacterial artificial chromosome-based gene transformation.
13:37
Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
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Cited by 7 •
2011
We demonstrate how hairy root composite plants can be used to study plant-rhizobium interactions and nodulation in the difficult-to-transform species Medicago truncatula.
10:12
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
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Cited by 21 •
2016
A high-throughput, automated, tobacco protoplast production and transformation methodology is described. The robotic system enables massively parallel gene expression and discovery in the model BY-2 system that should be translatable to non-model crops.
12:19
Bacterial Transformation: Electroporation
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2023
The term “transformation” refers cellular ingestion of foreign DNA. In nature, transformation can occur in certain types of bacteria. In molecular biology, however, transformation is artificially induced through the creation of pores in the bacterial cell walls. Bacterial cells that are able to take up DNA from the environment are called competent cells. Electrocompetent cells can be produced in the laboratory and transformation of these cells can be achieve via the application of an...
06:40
Plant Diversity - Concepts
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2019
From Water to Land Kingdom Plantae first appeared about 410 million years ago as green algae transitioned from water to land. Though challenging, this transition benefited early colonizers in several ways. Initially, most living organisms (including plants and animals) were ocean dwelling, making aquatic environments crowded and highly competitive. In contrast, land was a relatively uncolonized environment with ample resources and little to no predators or competitors. Terrestrial environments...
FAQs
In the common Agrobacterium-based approach, Agrobacterium acts as the biological transfer system that delivers a selected DNA sequence into plant cells. When that sequence becomes part of the plant genome, the introduced information can persist as the cells develop and contribute to a heritable trait change. This transfer step connects genetic design with selection and plant regeneration.
Selectable markers help researchers identify plant cells that received the introduced genetic material. This is important because only some treated cells may undergo the desired genetic change. After marker-based identification, researchers can focus tissue-culture efforts on those cells, improving the connection between DNA delivery and recovery of transformed plants rather than evaluating every treated cell equally.
Plant transformation provides a way to introduce genetic material whose effects can be examined in plant cells and regenerated plants. Researchers can use this approach to study what a gene does or to regulate how a gene is expressed, then relate the resulting trait changes to the introduced genetic information. This makes transformation useful for connecting molecular changes with biological function.
A common workflow begins by introducing a selected DNA sequence into plant cells, often using Agrobacterium. Researchers then use selectable markers to identify cells carrying the introduced material. Those selected cells undergo tissue culture, a controlled regeneration process that produces complete plants. The resulting plants can be examined for the intended genetic change and associated trait.
Researchers may apply plant transformation when they want crops with improved resistance, greater nutritional value, or better tolerance to environmental conditions. The method allows selected genetic material to be connected with specific trait goals and supports evaluation of the resulting plants. In agriculture and biotechnology, this makes it a tool for developing plants with characteristics useful for production or performance.
Beyond studying individual genes, plant transformation supports molecular breeding by providing a way to introduce and evaluate genetic information relevant to plant traits. It also enables the production of valuable proteins in plants, extending its use beyond crop improvement. These applications connect plant biology research with agricultural development and broader biotechnology programs.