Slow Growing Bacteria
Slow Growing Bacteria - Related Videos
03:26
Growing Magnetospirillum Bacteria and Assessing Their Magnetotactic Behavior
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2025
Source: Le Nagard, L., et al. Growing Magnetotactic Bacteria of the Genus Magnetospirillum: Strains MSR-1, AMB-1 and MS-1. J. Vis. Exp. (2018).This video demonstrates how to culture Magnetospirillum bacteria under microaerobic conditions using both liquid and semi-solid media. It highlights the formation of a visible bacterial band at the oxic-anoxic interface and its upward migration as oxygen is depleted. The video also shows how to assess magnetotactic behavior by observing directional...
08:00
Growing Crystals for X-ray Diffraction Analysis
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2023
Source: Laboratory of Dr. Jimmy Franco - Merrimack College X-ray crystallography is a method commonly used to determine the spatial arrangement of atoms in a crystalline solid, which allows for the determination of the three-dimensional shape of a molecule or complex. Determining the three-dimensional structure of a compound is of particular importance, since a compound's structure and function are intimately related. Information about a compound's structure is often used to explain its...
11:28
A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically
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Cited by 33 •
2015
Here, we present a protocol for determination of dalbavancin susceptibility of clinically relevant Gram-positive bacteria using a broth microdilution reference methodology.
11:45
Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
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Cited by 14 •
2018
Bacterial cells are spatially highly organized. To follow this organization over time in slow growing Myxococcus xanthus cells, a set-up for fluorescence live-cell imaging with high spatiotemporal resolution over several generations was developed. Using this method, spatiotemporal dynamics of important proteins for chromosome segregation and cell division could be determined.
02:05
Gene Evolution - Fast or Slow?
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2020
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences. In contrast, regions which code...
FAQs
Extended generation times often reflect a combination of low metabolic activity, scarce nutrients, complex growth requirements, and conditions that restrict energy production or cell division. These influences do not merely delay visible colonies; they determine which nutrients, temperature, and oxygen conditions will support detectable growth. Recognizing the cause helps investigators interpret delayed results rather than treating them as immediate evidence of absence.
Molecular detection can provide evidence of bacterial presence without requiring colonies to become visibly established. This is valuable when incubation may last days to weeks or when growth conditions are difficult to reproduce. Using molecular methods alongside culture helps investigators improve identification and detect organisms that might otherwise be overlooked because visible growth has not yet occurred.
Scarce nutrients can restrict the energy available for metabolism and cell division, while complex nutritional requirements may prevent growth on unsuitable media. Temperature and oxygen conditions also determine whether the organism can grow detectably. Matching these variables to the organism's requirements improves the chance of colony formation and helps distinguish unsuitable culture conditions from genuinely low or absent bacterial growth.
A practical investigation begins by selecting media suited to the organism's growth requirements, then establishing appropriate temperature and oxygen conditions. Samples must be incubated long enough for colonies to appear, which may require days to weeks. Because visible growth can be delayed, investigators may add molecular detection methods to support identification while incubation continues.
Studying these organisms can reveal microbial diversity that routine, short incubation periods may overlook. Their detection also supports investigations of nutrient cycling, because organisms with extended growth may remain part of environmental microbial communities even when they are difficult to recover rapidly. Combining prolonged incubation with molecular detection broadens the biological picture obtained from environmental samples.
Their extended growth requirements can complicate bacterial identification in investigations of infection, because visible colonies may not appear quickly. Prolonged incubation and molecular detection can help address that delay. These organisms are also relevant to antimicrobial-response studies, where researchers need to examine how slowly developing bacterial populations respond under appropriately chosen culture conditions.