Submerged Liquid-phase Bioreactor
Submerged Liquid-phase Bioreactor - Related Videos
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Liquid Phase Reactor: Sucrose Inversion
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2023
Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Both batch and continuous flow reactors are used in catalytic reactions. Packed beds, which use solid catalysts and a continuous flow, are the most common configuration. In the absence of an extensive recycle stream, such packed bed reactors are typically modeled as "plug flow". The other most common continuous reactor is a stirred tank, which is assumed to be perfectly...
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Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
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Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers
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2015
Here, we present a protocol to investigate multi-component phase diagrams using externally controlled magnetic beads as liquid carriers in a lab-in-tube approach. This approach can aid in applications that seek to gather further information on phase change in complex liquid systems.
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Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
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Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Cited by 32 •
2011
Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.
FAQs
Agitation keeps cells, nutrients, and heat more evenly distributed throughout the liquid, reducing gradients within the vessel. When the culture or reaction requires aerobic conditions, aeration supplies oxygen and removes carbon dioxide. Together, these operations help maintain an environment in which biological activity can proceed more consistently, making mixing and gas transfer central to process performance.
Sensors and automated controls help regulate pH, temperature, dissolved oxygen, and mixing rather than leaving these conditions to fluctuate uncontrolled. Continuous attention to these variables supports reproducible cultivation and biochemical reactions. It also gives researchers a practical way to maintain defined operating conditions when comparing experiments, optimizing a process, or extending work from laboratory studies toward larger-scale production.
These variables describe key conditions surrounding the biological material in the liquid medium. Regulating them provides a controlled basis for examining growth kinetics and metabolism, while also supporting consistent cultivation. Dissolved oxygen becomes especially important when aerobic growth is required, because aeration supplies oxygen and removes carbon dioxide as part of the controlled process.
A supported workflow begins with biological material or enzymes in a liquid nutrient medium, followed by controlled mixing through agitation. If aerobic activity is needed, aeration supplies oxygen and removes carbon dioxide. Sensors then monitor pH, temperature, dissolved oxygen, and mixing, while automated controls help maintain the selected conditions throughout cultivation or biochemical reaction work.
Applications include producing microbial biomass, enzymes, metabolites, vaccines, and other biological products. The same controlled platform can therefore serve processes centered on cell or microorganism cultivation as well as biochemical reaction work involving enzymes. Its value lies in connecting controlled liquid processing with product generation at both laboratory and industrial scales.
In biology and biotechnology, these systems provide a reproducible setting for studying growth kinetics, metabolism, and process optimization. Researchers can use the controlled environment to examine how cultivation behaves under regulated conditions, then apply the resulting understanding to bioprocess scale-up. This links laboratory investigation with development of larger production processes without changing the underlying liquid-phase approach.