Superficial Perineal Layer

The superficial perineal layer is the connective-tissue region immediately beneath the skin of the perineum, the area between the thighs that surrounds the urogenital and anal openings. It contains superficial fascia, muscles, vessels, nerves, and erectile tissues, while its fascial continuity with nearby abdominal and pelvic planes helps organize and support these structures. By enclosing tissues and providing attachment for muscles, the layer contributes to pelvic support, control of openings, and sexual function. Understanding its anatomy helps explain perineal injuries, guide surgical procedures, and predict how fluid, blood, or infection may spread through connected tissue planes.

Superficial Perineal Layer - Related Videos

Research

JoVE Journal - Medicine

Ultrasound Assessment of Flow-Mediated Dilation of the Brachial and Superficial Femoral Arteries in Rats

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Cited by 11 •

2016

Non-invasive assessment of endothelial function in humans can be determined by the flow-mediated dilation technique. Although thousands of studies have used this technique, no study has performed this technique non-invasively in rats. The following article describes non-invasive measurement of flow-mediated dilation in the brachial and superficial femoral arteries of rats.

Murine Superficial Lymph Node Surgery

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Cited by 10 •

2012

To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.

Education

JoVE Lab Manual - Chemistry

Thin-Layer Chromatography - Concepts

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2020

Chromatography Chromatography is a technique used in organic chemistry to separate compounds in a mixture based on their differences in solubility between two different phases. The concept is similar to liquid-liquid extraction, except in chromatography, the two phases consist of the stationary phase and the mobile phase. The stationary phase is a solid — typically a microscale hydrogel bead — while the mobile phase is a carrier solvent. In traditional chromatography, the stationary phase is...

In vivo Imaging of Deep Cortical Layers using a Microprism

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Cited by 11 •

2009

Right-angle microprisms inserted into the mouse neocortex allows for deep imaging of multiple cortical layers with a viewpoint typically found in slice. One-millimeter microprisms offer a wide field-of-view (~900 μm) and spatial resolutions sufficient to resolve dendritic spines. We demonstrate layer V neuronal imaging and neocortical vascular imaging using microprisms.

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

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Cited by 7 •

2015

The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.

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