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Chapters
- 01:35Part I: Microprism Optics and Microscope Setup
- 03:25Part II: Animal Surgery and Microprism Insertion
- 07:04Part III: Representative Results and Conclusion
Right-angle microprisms inserted into the mouse neocortex allows for deep imaging of multiple cortical layers with a viewpoint typically found in slice. One-millimeter microprisms offer a wide field-of-view (~900 μm) and spatial resolutions sufficient to resolve dendritic spines. We demonstrate layer V neuronal imaging and neocortical vascular imaging using microprisms.
Tags
In Vivo ImagingDeep Cortical LayersMicroprismDeep-brain Imaging ProbeReflective CoatingExcitation LightEmission LightSlice PreparationsField-of-viewNon-survival Surgical ProcedureMicroscope SetupLayer V Pyramidal NeuronsThy-1 YFP-H MiceApical DendritesTuftsDendritic SpinesFluorescent DyeBlood VesselsCortexLine-scanningRed Blood Cells (RBCs)CapillariesCellular StructuresCortical Function
