Upright Microscope Setup
Upright Microscope Setup - Related Videos
07:34
Upright Imaging of Drosophila Embryos
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Cited by 7 •
2010
Here we present a mounting protocol for stained Drosophila embryos in an upright position that allows imaging of cross-sections using Confocal microscopy.
12:29
Upright Imaging of Drosophila Egg Chambers
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Cited by 1 •
2015
The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.
09:47
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
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Cited by 13 •
2015
The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.
09:23
A Low Cost Setup for Behavioral Audiometry in Rodents
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Cited by 17 •
2012
A fast and inexpensive method for the behavioral determination of hearing parameters like hearing thresholds, hearing impairments or phantom perceptions (subjective tinnitus) is described. It uses pre-pulse inhibition of the acoustic startle response and can be easily implemented in a personal computer using a programmable AD/DA-converter and a piezo sensor.
06:15
Rapid Setup of Tissue Microarray and Tiled Area Imaging on the Multiplexed Ion Beam Imaging Microscope Using the Tile/SED/Array Interface
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Cited by 2 •
2023
Multiplexed ion beam imaging (MIBI) is often used to image tissue microarrays and tiled, contiguous tissue areas, but current software for setting up these experiments is cumbersome. The tile/SED/array interface is an intuitive, interactive graphical tool developed to dramatically simplify and accelerate MIBI run setup.
FAQs
Image formation depends on coordinated action of the objective and eyepiece lenses. The objective forms detail from the specimen, while the eyepiece enlarges the resulting image for viewing. Coarse focus makes larger positional adjustments, and fine focus refines the image after it approaches clarity. Using both controls in sequence helps produce a stable, sharper observation.
Illumination alignment determines whether light reaches the specimen in a useful and consistent way. Transmitted light is directed through the sample, whereas reflected light is directed onto it. Adjusting the illumination route along with stage position and focus helps preserve image clarity and stability, allowing biological structures to be observed more effectively.
Magnification and focus must be adjusted together because changing magnification changes the viewing conditions for the specimen. A suitable magnification supports observation of the needed biological detail, while coarse and fine focus controls refine the image at that setting. Coordinating these adjustments helps prevent unclear views and supports consistent examination.
A practical sequence begins by placing the specimen on the stage and positioning it for observation. Next, select an appropriate magnification, direct transmitted or reflected light as required, and adjust the stage position. Use coarse focus to approach an image, then apply fine focus and refine illumination until the view is clear and stable.
Choose transmitted illumination when light needs to pass through the specimen, and choose reflected illumination when light is directed onto its surface. The appropriate route depends on how the biological sample is presented for observation. Matching illumination to the specimen, then refining focus and stage position, improves the usefulness of the resulting image.
In biology, this configuration supports examination of cells, tissues, microorganisms, and prepared slides. It can serve teaching, routine analysis, and research because the operator can adjust magnification, illumination, stage position, and focus for different samples. The resulting clear and stable images help viewers inspect biological material in a controlled, repeatable manner.