Journal
/
Biology
/
Upright Imaging of Drosophila Embryos
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Upright Imaging of Drosophila Embryos

Upright Imaging of Drosophila Embryos

DOI:
10.3791/2175-v

•

07:34 min

•

September 13, 2010

•

, , , , , , ,
1Department of Biology,Case Western Reserve University, 2Department of Genetics,Case Western Reserve University

Chapters

  • 00:00Title
  • 00:53Introduction
  • 02:34Mounting Drosophila Embryos in Glycerin Jelly for Imaging
  • 04:28Cutting the Embryos
  • 05:25Visualization and Imaging of Embryos
  • 06:17Representative Images
  • 06:40Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a mounting protocol for stained Drosophila embryos in an upright position that allows imaging of cross-sections using Confocal microscopy.

Tags

Upright ImagingDrosophila EmbryosMorphogenetic GradientsCellular MovementsDorsal/ventral AxisCurrent TechniquesNew TechniqueMountingFixed EmbryosLabeled EmbryosCoronal ViewingConfocal ImagingGlycerin JellyEmbedding EmbryosJelly StripsUpright PositioningSandwiching EmbryosThin BeddingSlideAlignmentVisualizing EmbryosMicroscope StandPrecise MeasurementFluorescent Signals

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2175?article_type=v

Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos
check_url/2175?article_type=v

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
check_url/2175?article_type=v

Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures
check_url/2175?article_type=v

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
check_url/2175?article_type=v

Live Dissection of Drosophila Embryos: Streamlined Methods for Screening Mutant Collections by Antibody Staining
check_url/2175?article_type=v

In-vivo Centrifugation of Drosophila Embryos
check_url/2175?article_type=v

Primary Cell Cultures from Drosophila Gastrula Embryos
check_url/2175?article_type=v

RNAi Interference by dsRNA Injection into Drosophila Embryos
check_url/2175?article_type=v

Imaging Cell Shape Change in Living Drosophila Embryos
check_url/2175?article_type=v

Isolation and Purification of Kinesin from Drosophila Embryos

Read Article