Vacuum Sealing
Vacuum Sealing - Related Videos
04:36
Vacuum-Sealed Hot Water Bath Immersion for the Preparation of Anatomical and Surgical Cadaveric Bone Models
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Cited by 1 •
2022
The present protocol describes the maceration and cleaning of cadaveric bone with a vacuum-sealed, hot water bath immersion technique. This is a low-cost, safe, and effective method to produce anatomical specimens for surgical planning and medical education as an alternative to three-dimensional (3D) printed models.
06:20
Proper Operation of Vacuum Based Equipment
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2023
Source: Robert M. Rioux, Ajay Sathe & Zhifeng Chen, Pennsylvania State University, University Park, PA Vacuum is required for a number of laboratory procedures. This is most routinely achieved in the laboratory by the use of vacuum pumps. In addition to working at low pressures, vacuum pumps can also be used to enable rapid changing of the atmospheres in a reactor or flask by evacuation and backfilling.
04:41
Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures
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Cited by 7 •
2019
Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.
06:10
Accessible Silicone Chip-to-Membrane Sealing Procedure for Flexible, Reliable Bonding
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2026
A novel sealing protocol to produce water-tight silicone-based seals for membranes of Polydimethylsiloxane (PDMS) chip devices. Intended for labs setting-up chip models and small-scale chip platform development. We demonstrate the protocol using plastic and native tissue membranes. This procedure only requires PDMS, toluene, mold, membrane, and a vacuum chamber.
07:51
The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage
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Cited by 8 •
2010
The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.
FAQs
Removing air reduces the amount of oxygen surrounding the biological material. That lower exposure can slow oxidation, a chemical process that may damage or alter stored components, while the airtight barrier helps maintain a more stable package environment. The benefit is preservation support rather than complete protection, so vacuum sealing should be paired with suitable storage conditions.
Moisture control is a separate advantage from oxygen reduction. Limiting moisture exchange can help reduce desiccation, or unwanted drying, and can protect materials whose condition changes when water is lost or gained. However, the result depends on the packaging material and the quality of the seal. A compromised barrier can weaken both protections during storage or transport.
Vacuum sealing can slow the growth of many aerobic microorganisms because the package contains less oxygen, but it is not a sterilization step. Existing biological contamination is not thereby eliminated, and the package should not be treated as proof that a specimen, reagent, culture component, or food material is microbe-free. This distinction matters when planning storage and handling.
Temperature remains an important condition after sealing. The overview supports refrigerated or frozen storage when appropriate, because packaging alone does not determine preservation performance. Researchers should also consider whether the material and barrier tolerate the selected conditions. Matching temperature, packaging compatibility, and seal integrity helps maintain the intended state of stored biological materials.
At a basic workflow level, the material is placed in a compatible container or flexible barrier, air is removed, and the package is closed with an airtight seal. The finished package should then be checked for suitable seal integrity and kept under the appropriate refrigerated or frozen condition. These steps support preservation, but they do not replace material-specific storage decisions.
In biology, vacuum sealing is useful when specimens, reagents, culture components, laboratory-study foods, or other moisture-sensitive materials must be stored or transported. The expected outcome is reduced exposure to oxygen and moisture exchange, which can help preserve condition over time. Its value is greatest when the package, seal, and temperature are matched to the material.