Gas Liquid Interface

A gas-liquid interface is the boundary where a gas and a liquid meet, creating a distinct environment important for mass transfer, surface chemistry, and biological organization. Molecules at this boundary experience unbalanced cohesive forces, producing surface tension, while gases can dissolve, diffuse across the interface, and reach equilibrium according to concentration and pressure gradients. In biology, these principles govern oxygen and carbon dioxide exchange in lung alveoli, where pulmonary surfactants reduce surface tension and help prevent collapse. Studying gas-liquid interfaces also supports research on microbial communities, protein adsorption, aerosols, and systems that couple physical transport with cellular function.

Gas Liquid Interface - Related Videos

Research

JoVE Journal - Biology

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

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Cited by 135 •

2013

Nasal epithelial cells, obtained through superficial scrape biopsy of human volunteers, are expanded and transferred onto tissue culture inserts. Upon reaching confluency, cells are grown at air liquid interface, yielding cultures of ciliated and non-ciliated cells. Differentiated nasal epithelial cell cultures provide viable experimental models for studying the respiratory mucosa.

Establishing a Co-Culture Model to Study Host-Pathogen Interactions at the Air-Liquid Interface

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2026

Source: Montefusco-Pereira, C. V. et al. P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives. J. Vis. Exp.(2020)This video demonstrates a co-culture model of bronchial epithelial cells and macrophage-like cells at the air-liquid interface to study host-pathogen interactions. Pathogenic bacterial infection compromises epithelial membrane integrity, triggering macrophage-like cell activation and...

Viral Growth in Human Nasal Epithelial Cells Cultured at an Air-Liquid Interface

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2026

Source: Otter, C. J., et al. Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions. J. Vis. Exp. (2023)This video demonstrates the growth of coronavirus in human nasal epithelial cells cultured at an air-liquid interface, mimicking infection and viral replication in the human nasal cavity.

Education

JoVE Science Education - Engineering

Gas Absorber

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2023

Source: Michael G. Benton and Kerry M. Dooley, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Gas absorbers are used to remove contaminants from gas streams. Multiple designs are used to accomplish this objective1. A packed bed column uses gas and liquid streams running counter to each other in a column packed with loose packing materials, such as ceramics, metals, and plastics, or structured packing1. The packed bed uses surface area created by the packing to...

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

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Cited by 67 •

2011

Pulmonary epithelial cells can be isolated from the respiratory tract of mice and cultured at air-liquid interface as a model of differentiated respiratory epithelium. A protocol is described for isolating, culturing and exposing these cells to mainstream cigarette smoke, in order to study molecular responses to this environmental toxin.

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