White Adipose Tissue
White Adipose Tissue - Related Videos
03:28
White and Brown Adipose Depot Collection from Mouse Pup: A Surgical Procedure to Harvest the White Adipose Tissue and Brown Adipose Tissue from Mouse Pup
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2025
In this video, we demonstrate the surgical procedure to isolate the subcutaneous white adipose tissue and interscapular brown adipose tissue from a newborn mouse.
06:19
Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
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Cited by 19 •
2018
The focus of the present study is to demonstrate the whole-mount immunostaining and visualization technique as an ideal method for 3D imaging of adipose tissue architecture and cellular component.
06:39
Fat-Covered Islet Transplantation Using Epididymal White Adipose Tissue
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Cited by 3 •
2021
This fat-covered islet transplantation method is suitable for the detection of engrafted islets in the intraperitoneal cavity. Notably, it does not require the use of biobinding agents or suturing.
08:14
A Microphysiologic Platform for Human Fat: Sandwiched White Adipose Tissue
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Cited by 7 •
2018
White adipose tissue (WAT) has critical deficiencies in its current primary culture models, hindering pharmacological development and metabolic studies. Here, we present a protocol to produce an adipose microphysiological system by sandwiching WAT between sheets of stromal cells. This construct provides a stable and adaptable platform for primary WAT culture.
06:08
Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
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Cited by 2 •
2023
This protocol describes the semi-automated isolation of the stromal vascular fraction (SVF) from murine adipose tissue to obtain preadipocytes and achieve adipocyte differentiation in vitro. Using a tissue dissociator for collagenase digestion reduces experimental variation and increases reproducibility.
FAQs
Insulin favors an energy-storage state by promoting glucose uptake and triglyceride formation in adipocytes. During fasting or stress, catecholamines shift the tissue toward fuel mobilization by activating lipolysis, the breakdown of stored triglycerides. This opposing hormonal control allows white adipose tissue to adjust fatty-acid availability according to the body's nutritional and physiological demands.
Adipokines allow white adipose tissue to communicate with systems that regulate appetite, insulin sensitivity, inflammation, and energy balance. Their signaling makes adipose tissue an active participant in whole-body physiology rather than a passive lipid depot. Studying these secreted factors helps connect adipose biology with metabolic disorders, including obesity and type 2 diabetes.
During energy surplus, the large, unilocular adipocytes characteristic of WAT enlarge as triglyceride stores increase. Research on remodeling examines how this tissue changes as its cells and regulatory interactions respond to altered energy balance. These changes are relevant to understanding how excess adipose accumulation may contribute to conditions such as obesity, insulin resistance, and cardiovascular disease.
A comprehensive study considers adipose development, tissue remodeling, endocrine signaling, and communication with immune and metabolic systems. Examining these relationships helps reveal how WAT influences processes beyond lipid storage and how its behavior changes in disease-associated states. This systems-level perspective is important when evaluating potential metabolic therapies or designing adipose tissue engineering strategies.
WAT research connects changes in adipocyte size, lipid storage, hormonal signaling, and tissue interactions with metabolic disease. Because the tissue influences insulin sensitivity, inflammation, appetite, and energy balance, its dysfunction can be studied as part of the biological context surrounding obesity and type 2 diabetes. Findings may also support the development of metabolic therapies.
Investigating WAT contributes to cardiovascular disease research by examining how adipose-derived signals and metabolic regulation relate to broader disease processes. Adipokines influence inflammation and insulin sensitivity, while changes in energy storage and lipid mobilization affect systemic metabolism. These connections make white adipose tissue a relevant biological system for exploring cardiovascular risk in the context of metabolic dysfunction.
White adipose tissue provides a research context for tissue engineering because its development and remodeling can be studied alongside endocrine, immune, and metabolic interactions. Understanding these properties may help inform strategies aimed at creating or modifying adipose tissue. The goal is not only to reproduce its structure, but also to consider the biological signaling that gives the tissue systemic relevance.