Yeast Transformation
Yeast Transformation - Related Videos
08:30
Yeast Transformation and Cloning
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2023
S. cerevisiae are unicellular eukaryotes that are a commonly-used model organism in biological research. In the course of their work, yeast researchers rely upon the fundamental technique of transformation (the uptake of foreign DNA by the cell) to control gene expression, induce genetic deletions, express recombinant proteins, and label subcellular structures. This video provides an overview of how and why yeast transformation is carried out in the lab. The important features of yeast...
05:34
Oral Delivery of Transformed Yeast Cells into the Mouse Intestinal Immune System
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2025
This video showcases the oral administration of transformed yeast cells into a mouse model. The transformed yeast cells are orally administered using a gavage technique, reaching the mouse's stomach. Later, the cells migrate to the small intestine, where microfold cells engulf them and facilitate their transfer to Peyer's patches and gain access to the intestinal immune system.
12:12
Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
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Cited by 2 •
2016
Presented here is a unified description of techniques that can be used to develop, transform, administer, and test heterologous protein expression of the probiotic yeast Saccharomyces boulardii.
05:20
Yeast Two-Hybrid Assay to Determine Protein Self-Association in Yeast Cells
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2025
This video demonstrates the yeast two-hybrid assay to detect specific protein self-interactions. In this assay, the protein of interest is tagged to two domains of a transcription factor that encodes the β-galactosidase enzyme. When the two proteins interact, this causes transcription and translation to form the enzyme, which is then detected using a biochemical assay.
08:57
High-throughput Yeast Plasmid Overexpression Screen
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Cited by 12 •
2011
Here we describe a plasmid overexpression screen in Saccharomyces cerevisiae, using an arrayed plasmid library and a high-throughput yeast transformation protocol with a liquid handling robot.
FAQs
In the common chemical approach, lithium acetate and polyethylene glycol act before heat shock. Together, they help plasmid DNA associate with the yeast cell surface, creating conditions in which the subsequent heat treatment can promote DNA uptake. This sequence links chemical preparation with a physical uptake step, rather than treating heat shock as an independent transformation mechanism.
A selectable marker links successful DNA uptake to cell growth under a defined selection. After the transformation treatment, cells are placed on selective medium, where the marker permits transformed cells to grow. This enrichment identifies cells carrying the introduced plasmid and allows researchers to distinguish them from cells that did not acquire the foreign DNA.
Yeast is valuable because it combines relatively simple culture requirements with conserved cellular processes in a eukaryotic model organism. DNA introduced into yeast can therefore support investigations of gene function, metabolic pathways, or protein production in a eukaryotic setting. This makes the system useful for connecting molecular manipulation with broader biological interpretation.
A typical workflow begins by combining yeast cells with plasmid DNA in the presence of lithium acetate and polyethylene glycol. Heat shock then promotes DNA uptake. The cells are subsequently placed on selective medium, where the selectable marker allows transformed cells to grow. This sequence connects DNA delivery with selection of cells carrying the introduced material.
Yeast transformation supports several experimental goals. Gene cloning uses yeast as a host for introduced genetic material, heterologous protein expression examines production of proteins from another source, functional genomics investigates gene roles, and synthetic biology develops engineered biological systems. The method consequently supports both gene-focused studies and deliberate modification of cellular pathways.
By introducing selected DNA into yeast, researchers can examine how genetic material relates to gene function and metabolic pathways. The eukaryotic context is important because yeast retains conserved cellular processes while remaining comparatively simple to culture. Results from transformed cells can therefore help connect a specific genetic change with broader cellular or pathway-level behavior.