Fixation Dehydration
Fixation Dehydration - Related Videos
01:15
Dehydration Synthesis
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2026
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.Synthesis of carbohydratesSugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from one reactant...
10:21
Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation
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Cited by 3 •
2017
Fluorescence in-situ hybridization (FISH) is often required in combination with histopathology and molecular diagnostics for selection of therapy in personalized medicine. A novel non-cross-linking, formalin-free tissue fixative that allows high quality morphologic, molecular and FISH analyses from the same specimen by addition of a post-fixation step before FISH is presented.
02:35
Acid-Catalyzed Dehydration of Alcohols to Alkenes
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2025
In a dehydration reaction, a hydroxyl group in an alcohol is eliminated along with the hydrogen from an adjacent carbon. Here, the products are an alkene and a molecule of water. Dehydration of alcohols is generally achieved by heating in the presence of an acid catalyst. While the dehydration of primary alcohols requires high temperatures and acid concentrations, secondary and tertiary alcohols can lose a water molecule under relatively mild conditions. The acid-catalyzed dehydration of...
08:53
Whole Animal Perfusion Fixation for Rodents
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Cited by 531 •
2012
Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.
01:03
Fixation and Sectioning
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2023
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens. The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
FAQs
Fixation and dehydration solve different preservation problems within one workflow. Fixation stabilizes cellular structures by cross-linking or precipitating tissue components, limiting deterioration during processing. Dehydration then replaces remaining water with progressively stronger alcohol solutions. Keeping these functions distinct helps preserve structural detail while preparing the specimen for later clearing, paraffin infiltration, sectioning, and staining.
Stepwise alcohol changes allow water to be replaced gradually rather than abruptly. This staged transition helps reduce distortion in the specimen and supports more consistent tissue morphology. The concentration sequence is therefore a key processing condition: it links effective water removal with preservation of cellular organization needed for dependable microscopic examination.
Fixation chemistry determines how tissue components are stabilized before water removal begins. Cross-linking preserves structures by linking components, whereas precipitation stabilizes them by precipitating tissue components. Because both mechanisms act on the specimen before graded alcohol processing, the quality of fixation affects how well cellular detail remains available for later microscopy.
Once water has been removed, the tissue can proceed to clearing and paraffin infiltration. Clearing prepares the dehydrated specimen for the next processing step, while paraffin infiltration supports subsequent sectioning. The processed tissue can then be cut into sections and stained, creating a practical route from preserved specimen to microscopic evaluation.
It is useful when researchers need tissue organization to remain sufficiently intact for examination rather than deteriorate after collection. The workflow supports histology, pathology, and biological research involving tissue structure. By producing specimens compatible with sectioning and staining, it enables consistent preparation for light microscopy and, within the described workflow, other imaging methods.
Preserving cellular and tissue structure allows investigators to examine spatial organization rather than only isolated or degraded components. Sections produced after clearing and paraffin infiltration can be stained for microscopic assessment, making structural detail accessible across prepared specimens. This consistency is especially valuable when histological or pathological observations depend on comparing tissue organization.