Zebrafish Laboratory
Zebrafish Laboratory - Related Videos
11:47
Regular Care and Maintenance of a Zebrafish (Danio rerio) Laboratory: An Introduction
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Cited by 320 •
2012
This protocol outlines regular maintenance and care to maintain optimal conditions for zebrafish husbandry. The video illustrates the protocol for system maintenance, regular housing, feeding, breeding, and raising of zebrafish larvae.
06:55
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
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Cited by 42 •
2011
A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.
07:58
Zebrafish Maintenance and Husbandry
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2023
The zebrafish (Danio rerio) is a powerful vertebrate model system for studying development, modeling disease, and screening for novel therapeutics. Due to their small size, large numbers of zebrafish can be housed in the laboratory at low cost. Although zebrafish are relatively easy to maintain, special consideration must be given to both diet and water quality to in order to optimize fish health and reproductive success. This video will provide an overview of zebrafish husbandry and...
06:19
Decontamination for Laboratory Biosafety
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2023
Robert M. Rioux and Zhifeng Chen, Pennsylvania State University, University Park, PA Decontamination is essential for laboratory biosafety, as the accumulation of microbial contamination in the laboratory can lead to the transmission of disease. The degree of decontamination can be classified as either disinfection or sterilization. Disinfection aims to eliminate all pathogenic microorganisms, with the exception of bacterial spores on lab surfaces or equipment. Sterilization, on the other hand,...
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
FAQs
External fertilization gives researchers access to embryos outside the parent, making early development available for direct study. Because these embryos develop rapidly and remain transparent during early stages, investigators can observe organ formation as it occurs rather than relying only on later biological outcomes. This supports in vivo analysis of developmental processes under controlled laboratory conditions.
Transparency allows researchers to observe developing structures directly during early life stages. In a zebrafish laboratory, this visual access supports examination of organ formation and other developmental changes while they occur, linking visible biological outcomes with experimental manipulation. The approach is especially useful when researchers need to assess how altered gene function or physiological conditions affect development.
Manipulating gene function enables researchers to examine how particular genetic activities contribute to biological processes. When combined with rapid development and transparent embryos, the method can connect changes in gene function with effects on organ formation or other observable outcomes. This makes zebrafish useful for investigating genetic mechanisms relevant to normal biology and disease.
Conserved cellular and molecular pathways provide a biological basis for comparing zebrafish findings with human processes. Results from experiments can therefore reveal general biological principles or suggest disease-related mechanisms, while still requiring further investigation before therapeutic conclusions are drawn. This cross-species relevance helps connect laboratory observations to questions in human biology.
Researchers can observe organ formation, investigate altered gene function, and assess physiological responses under controlled conditions. These outcomes allow experiments to address developmental biology, genetics, and biological responses to tested conditions. The combination of direct observation and controlled assessment helps investigators relate an experimental change to a specific developmental, genetic, or physiological result.
Applications include developmental biology, genetics, regeneration, disease mechanisms, toxicology, and drug discovery. In disease-oriented work, findings may clarify mechanisms associated with illness, whereas drug-discovery studies can help prioritize potential therapeutic compounds for further investigation. Toxicology and physiological-response experiments extend the model's use to evaluating how biological systems respond to controlled exposures or conditions.