Drg Embedding
Drg Embedding - Related Videos
03:44
Co-Culture of Schwann Cells with DRG Neurons on a Pre-Stretched Membrane
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2025
The video demonstrates the co-culturing of dorsal root ganglion (DRG) neurons with Schwann cells. DRG neurons are first grown on a pre-stretched membrane for aligned axon growth. Schwann cells are then introduced, which attach to the axons, insulating them and promoting neural cell survival and function.
02:49
Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model
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2023
In this video, we demonstrate the extraction of dorsal root ganglion or DRG from a harvested rat spine for downstream applications.
09:14
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
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Cited by 20 •
2011
The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.
04:25
Isolation of DRG Neurons and Coculture with Schwann Cell Precursors to Generate Schwann Cells
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2025
This video demonstrates a procedure for isolating dorsal root ganglion (DRG) neurons from rat embryos and co-culturing them with Schwann cell precursors to generate mature Schwann cells. The isolated DRGs are processed to isolate neurons and glial cells. The isolated cells are cultured to purify the neurons, which are then co-cultured with Schwann cell-like cells (SCLCs) in a co-culture medium. Interaction with the DRG neurons leads to the maturation of SCLCs into Schwann cells.
09:04
Yeast Colony Embedding Method
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Cited by 7 •
2011
A method for embedding yeast colonies allowing sectioning for light and electron microscopy. This protocol allows determination of the distribution of sporulated cells and pseudohyphal cells within colonies providing a new tool toward understanding the organization of cell types within a fungal community.
FAQs
The biomaterial matrix provides a defined extracellular environment through which neurons and supporting cells extend neurites. Because researchers can observe outgrowth within this three-dimensional setting, they can examine how nearby cancer cells, secreted factors, or therapeutic compounds alter neural growth and invasion. The matrix therefore connects controlled experimental conditions with tissue interactions relevant to cancer biology.
Drg Embedding can be used to test whether cancer cells or their secreted factors stimulate, redirect, or otherwise influence neurite growth. These experiments help researchers investigate neurotrophic signals, meaning signals that affect neural growth or maintenance, in the tumor environment. Comparing responses under controlled co-culture conditions can reveal communication pathways that may contribute to tumor innervation.
A three-dimensional setup allows neurites to extend through a defined matrix while interacting with nearby tissues or tumor cells. This arrangement supports direct observation of growth patterns and neural invasion rather than examining cancer cells or neurons in isolation. Its physiologically relevant organization makes the approach useful for connecting cellular behavior with tumor innervation and cancer-associated pain mechanisms.
Researchers can vary the presence of cancer cells, the exposure to cancer-derived secreted factors, or the addition of therapeutic compounds. They can then monitor how these conditions affect neurite extension and neural invasion within the matrix. This controlled design helps separate effects caused by tumor-associated signals from responses associated with candidate treatments or other experimental conditions.
A typical workflow places dorsal root ganglia within a biomaterial matrix, establishes the relevant cancer-cell, factor, or compound condition, and follows neurite extension using accessible imaging. Co-culture experiments can position neural tissue near tumor cells or expose it to their secreted signals. The resulting images provide a basis for comparing neural growth and invasion across conditions.
Researchers may select Drg Embedding when they need to examine communication between neural tissue and tumors in a controlled three-dimensional culture. The method is suited to studies of tumor innervation, cancer-nerve interactions, and mechanisms of pain. It can also support evaluation of therapeutic compounds and help identify neurotrophic signals or potential treatment targets.
Imaging can document neurite growth through the matrix and reveal changes associated with cancer cells, secreted factors, or therapeutic compounds. These observations can indicate whether tumor-related conditions promote neural invasion or alter neural outgrowth. In cancer research, the findings may help prioritize neurotrophic signals for further study and evaluate targets relevant to tumor innervation or pain.