Intracisternal Injection
Intracisternal Injection - Related Videos
05:55
Induction of Leptomeningeal Cells Modification Via Intracisternal Injection
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Cited by 1 •
2020
We describe an intracisternal injection that employs a needle bent at the tip that can be stabilized to the skull, thus eliminating the risk of damage to the underlying parenchyma. The approach can be used for genetic fate mapping and manipulations of leptomeningeal cells and for tracking cerebrospinal fluid movement.
03:37
Intracisternal Delivery of Meningococci into Mouse Models to Induce Meningococcal Meningitis
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2025
In this video, we demonstrate the intracisternal delivery of Neisseria meningitidis into a mouse model to generate meningitis infection.
10:03
Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery
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Cited by 3 •
2019
Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues.
04:10
Surgical Cisterna Magna Injection: A Method for Administering Tumor Cells Directly into Central Nervous System of Murine Model
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2023
In this video, we describe the procedure to deliver human-derived tumor cells into the cisterna magna of a mouse’s brain. The orthotopic mouse model can be used to study metastatic spread of cancer to the cranial meninges.
08:28
Zebrafish Brain Ventricle Injection
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Cited by 72 •
2009
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
FAQs
The cisterna magna route places a test material directly into cerebrospinal fluid, so investigators can examine distribution around the brain and spinal cord without depending as heavily on passage from the bloodstream across the blood-brain barrier. This is useful when evaluating whether a candidate treatment can reach relevant central nervous system tumor environments.
Injection volume determines how much material enters the cerebrospinal fluid, while needle placement determines whether the intended space receives that material. Inconsistent control of either factor can make treatment exposure and distribution difficult to compare between animals. Careful standardization therefore supports reliable experimental results and helps reduce procedure-related risks.
Intracisternal Injection reduces reliance on a substance crossing the blood-brain barrier after systemic administration. Instead, the material enters cerebrospinal fluid, where it can circulate around the brain and spinal cord. This distinction allows cancer researchers to focus specifically on central nervous system delivery and to assess candidate treatments under conditions that address access limitations.
The procedure is typically performed with the subject under anesthesia. A fine needle is positioned at the cisterna magna, and a measured volume of the selected material is introduced into the cerebrospinal fluid. Accurate placement and controlled delivery are central procedural requirements because both influence distribution, reproducibility, and animal safety.
In cancer research, this technique can support studies of central nervous system tumors by testing how candidate drugs are delivered into cerebrospinal fluid. Investigators can use it to evaluate therapeutic responses and to model how treatments distribute around the brain and spinal cord, providing information relevant to treatment access and activity in the central nervous system.
Researchers can assess two connected outcomes: how a candidate treatment distributes within cerebrospinal fluid and whether it produces a therapeutic response in central nervous system tumor studies. Because the material circulates around the brain and spinal cord, the method helps link delivery behavior with treatment evaluation rather than examining response without considering central nervous system access.