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Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery
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Immunology and Infection
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JoVE Journal Immunology and Infection
Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

DOI:
10.3791/60047-v

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10:03 min

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November 05, 2019

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, , , , , , , ,
1Department of Molecular Medicine and Medical Biotechnology,University of Naples Federico II, 2Department of Science and Technology,Sannio University, 3CEINGE – Advanced Biotechnology

Chapters

  • 00:05Title
  • 01:48Preparation of Bacteria for In Vivo Infection Studies
  • 03:09Intra-cisternal Injection of Mice
  • 05:07Evaluation of Colony Forming Units (CFU) Counts in Peripheral Organs
  • 06:39Preparation of Brain Tissues for CFU Count
  • 08:05Results: Survival of Mice Following Intracisternal Injection of Wild-type and Mutant N. meningitidis
  • 09:28Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues.

Tags

Keywords: Neisseria MeningitidisMeningococcal MeningitisSerogroup CMouse ModelIntracisternal InoculationHost-pathogen InteractionPathogenesisTherapeutic StrategyPassive Immunotherapy

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