True Fisp Sequence
True Fisp Sequence - Related Videos
01:28
True Stress and True Strain
0 Views •
2024
Engineering stress is calculated as the load divided by the original, undeformed cross-sectional area. It approximates a material under load. This approximation is especially relevant post-yield in ductile materials. Though engineering stress-strain diagrams are often used for their convenience and accessibility, they can sometimes fall short in accuracy, particularly when dealing with large strain values. In contrast, true stress offers a more precise portrayal. It is computed by dividing the...
09:40
Detection of True IgE-expressing Mouse B Lineage Cells
0 Views •
Cited by 3 •
2014
In vitro analysis of class switch recombination in mice is challenging due to cytophilic IgE molecules bound to Fc receptors on the surface of B cells. We describe a method for IgE detection using trypsin-mediated cleavage of surface-bound IgE prior to fixation and permeabilization for cytoplasmic fluorescence staining.
08:57
Amplicon Sequencing using the Long-Read Sequencing Technologies
0 Views •
2025
This protocol was optimized for targeted deep sequencing of 18 drug-resistance regions in Mycobacterium tuberculosis using a long-read sequencing platform, followed by analysis with a tuberculosis-specific bioinformatics pipeline designed for long-read data.
14:06
Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
0 Views •
Cited by 10 •
2012
Pooled DNA sequencing is a fast and cost-effective strategy to detect rare variants associated with complex phenotypes in large cohorts. Here we describe the computational analysis of pooled, next-generation sequencing of 32 cancer-related genes using the SPLINTER software package. This method is scalable, and applicable to any phenotype of interest.
09:18
TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
0 Views •
Cited by 7 •
2016
Here we present a protocol for screening of a heptamer-type sgRNA library for potential therapeutic drugs against blood cancers.
FAQs
Balancing the gradient areas in the slice, phase, and frequency directions prevents net gradient effects from accumulating between successive radiofrequency excitations. This allows transverse magnetization to persist rather than being fully discarded after each repetition. Over repeated very short intervals, the magnetization reaches a steady state, supporting efficient signal formation and the sequence’s rapid image acquisition.
The steady-state signal retains transverse magnetization across repeated acquisitions, creating bright signal from fluid-containing structures relative to surrounding tissues. This difference improves visual separation of anatomy and can make lesion boundaries easier to inspect when tumors are adjacent to fluid or fluid-rich structures. The resulting contrast is particularly useful in abdominal, pelvic, and cardiac imaging.
TrueFISP acquires images rapidly through very short repetition intervals, so it can capture anatomy during changing motion and support dynamic studies. At the same time, moving structures can alter the steady-state signal and image appearance. Its speed may therefore help reduce motion-related degradation, while also making motion-sensitive imaging useful for evaluating anatomy that changes over time.
The scanner repeatedly applies the sequence with very short repetition intervals while balancing gradient areas in all three spatial directions. Transverse magnetization is carried from one repetition to the next until a steady state develops, and the resulting signals form images with strong fluid-to-tissue contrast. This rapid repeated acquisition can be adapted for motion-sensitive or dynamic examinations.
In cancer research, the sequence can support noninvasive visualization of anatomy, fluid-containing structures, and lesion boundaries. Its reported applications include abdominal, pelvic, and cardiac MRI, where clear fluid-to-tissue contrast may help show tumor location in relation to nearby structures. Researchers can also use its rapid acquisition capability when studying changing anatomy or dynamic processes.
The sequence can provide repeated, noninvasive views of lesion location and surrounding anatomy, including in dynamic studies. Comparing these images during research assessments may help characterize changes associated with treatment response, while the high signal-to-noise ratio and fluid-to-tissue contrast support lesion visualization. Faster acquisition can also limit motion-related image degradation, improving the consistency of serial imaging.