Tumor Chip
Tumor Chip - Related Videos
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
01:35
Organ-on-Chip Models
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2026
Introduction to Organ-on-Chip Models Organ-on-chip models are advanced microphysiological systems that recreate key structural and functional features of human organs in a controlled laboratory setting. These devices integrate living human cells within microfluidic channels, allowing researchers to simulate tissue–tissue interfaces, fluid flow, and mechanical forces that are essential for normal organ function. Unlike traditional cell cultures, which often lack these dynamic physiological cues,...
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
05:58
Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
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Cited by 3 •
2023
The clinical microfluidic chip is an important biomedical analysis technique that simplifies clinical patient blood sample preprocessing and immunofluorescently stains circulating tumor cells (CTCs) in situ on the chip, allowing the rapid detection and identification of a single CTC.
12:43
On-Chip Endothelial Inflammatory Phenotyping
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Cited by 3 •
2012
Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.
FAQs
Fluid flow through microfluidic channels creates spatial differences in nutrients, oxygen, and drug exposure. These gradients can alter how tumor cells grow and respond to treatment, making it possible to examine effects under conditions that vary across the engineered tissue rather than applying one uniform environment. This helps connect treatment response with local microenvironment.
The extracellular matrix provides a surrounding scaffold for tumor cells, while stromal or immune cells, when included, allow researchers to examine interactions beyond tumor cells alone. These elements can influence tumor behavior and treatment response within the chip. Including them therefore helps model communication between malignant cells and their surrounding tissue.
Conventional cultures generally provide fewer opportunities to reproduce three-dimensional tissue organization, controlled fluid flow, and interactions with extracellular matrix or additional cell types. A Tumor Chip combines these features under tunable conditions, allowing researchers to study tumor growth, invasion, and treatment response in a more physiologically relevant setting than conventional culture alone.
Researchers can vary nutrient, oxygen, and drug conditions within the chip to observe how tumor cells behave under different stresses. Comparing growth and treatment responses across these settings can reveal how environmental differences relate to reduced drug sensitivity or resistance. The controlled design also supports testing specific conditions without changing the entire experimental system.
Researchers establish living tumor cells within microfluidic channels alongside extracellular matrix and, when appropriate, stromal or immune cells. They then maintain controlled fluid flow, adjust conditions such as nutrient, oxygen, or drug exposure, and monitor outcomes including growth, invasion, cell interactions, or treatment response. This workflow links engineered conditions to measurable cancer behaviors.
A chip can maintain tumor cells in a controlled environment while researchers expose them to anticancer drugs under tunable flow and gradient conditions. They can then assess changes in tumor growth and treatment response, including responses associated with differing oxygen, nutrient, or drug exposure. This approach supports drug testing in a model that includes relevant tissue features.
Connecting the engineered model with patient-derived cells may allow researchers to examine how an individual’s tumor responds to candidate treatments under controlled conditions. The resulting response information could support more personalized treatment selection. Patient-linked testing also contributes to research goals that include improving physiological relevance and reducing reliance on animal studies.