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On-Chip Endothelial Inflammatory Phenotyping
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Bioengineering
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JoVE Journal Bioengineering
On-Chip Endothelial Inflammatory Phenotyping

On-Chip Endothelial Inflammatory Phenotyping

DOI:
10.3791/4169-v

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12:43 min

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July 21, 2012

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, , , , , ,
1Department of Biomedical Engineering,University of California, Davis

Chapters

  • 00:05Title
  • 03:04Substrate Preparation and Cell Culture
  • 04:30Fabrication of PDMS VMMC
  • 06:37Cell Shearing
  • 08:30Monocyte Adhesion Assay
  • 10:55Representative Results
  • 11:27Conclusion

Summary

Automatic Translation

Automatic Translation

Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.

Tags

On-Chip Endothelial Inflammatory PhenotypingAtherogenesisMetabolic AbnormalitiesSystemic InflammationEndothelial DysfunctionFunctional Changes In EndotheliumRisk AssessmentTherapeutic StrategyRegulation Of InflammationLocal HemodynamicsNon-random Spatial Distribution Of AtherosclerosisLab-on-a-chipQuantitatively Assay Metabolic PerturbationInflammatory Events In Endothelial CellsMonocytesFlow ConditionsSoft LithographyMicrofabricate Vascular Mimetic Microfluidic Chambers (VMMC)Cultured EC MonolayersImaging Inflammatory EventsShear FieldCytokine-induced InflammationLipid-induced InflammationRAGE-induced InflammationHuman Aortic EC (HAEC)Monocytic Cell Rolling And ArrestTNF-alpha

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