Vacuum Chamber Operation
Vacuum Chamber Operation - Related Videos
06:20
Proper Operation of Vacuum Based Equipment
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2023
Source: Robert M. Rioux, Ajay Sathe & Zhifeng Chen, Pennsylvania State University, University Park, PA Vacuum is required for a number of laboratory procedures. This is most routinely achieved in the laboratory by the use of vacuum pumps. In addition to working at low pressures, vacuum pumps can also be used to enable rapid changing of the atmospheres in a reactor or flask by evacuation and backfilling.
07:57
Operating the Glovebox
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2023
Robert M Rioux, Ajay Sathe, Zhifeng Chen, Pennsylvania State University, University Park, PA A glovebox is an isolated enclosure that is designed to maintain an inert atmosphere. The manipulation of chemicals or apparatus is done via the use of gloves, which allow operation while still maintaining an inert atmosphere. A positive pressure is utilized to compensate for any passive leaks. Objects can be transferred in and out of the box using an antechamber that acts as an airlock between the box...
08:32
Tracking Rats in Operant Conditioning Chambers Using a Versatile Homemade Video Camera and DeepLabCut
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Cited by 9 •
2020
This protocol describes how to build a small and versatile video camera, and how to use videos obtained from it to train a neural network to track the position of an animal inside operant conditioning chambers. This is a valuable complement to standard analyses of data logs obtained from operant conditioning tests.
07:49
Development of an Experimental Setup for the Measurement of the Coefficient of Restitution under Vacuum Conditions
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Cited by 11 •
2016
The coefficient of restitution is a parameter that describes the loss of kinetic energy during collision. Here, a free-fall setup under vacuum conditions is developed to be able to determine the coefficient of restitution parameter for particles in micrometer range with high impact velocities.
10:16
Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
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Cited by 39 •
2014
This method explains how to build and operate a continuous 13C and 15N isotope labeling chamber for uniform or differential plant tissue labeling. Representative results from metabolic and structural labeling of Andropogon gerardii are discussed.
FAQs
Lower pressure changes phase behavior by reducing the gas pressure opposing molecules leaving a liquid. As a result, solvents can boil or evaporate at lower temperatures than they would under atmospheric conditions. This mechanism supports concentration and drying while helping researchers study phase changes under controlled conditions. The practical outcome is solvent removal with reduced thermal demands.
A pump and pressure gauge serve different but complementary functions. The pump removes gas from the vessel, whereas the gauge indicates the pressure achieved and helps track whether conditions remain stable. Sealed connections are essential because leaks can undermine the intended low-pressure environment. Monitoring these elements together improves control and makes chemical processing more reproducible.
Reduced gas density limits the amount of surrounding gas available to contact a sample. This can decrease exposure to oxygen or moisture, which is useful when handling air-sensitive materials. The chamber therefore provides a controlled environment for chemical work in which unwanted contact with atmospheric components is minimized, supporting more consistent sample preparation and processing.
The workflow begins by placing the material in the enclosed vessel and establishing sealed connections. A pump then extracts gas, while a gauge tracks the chamber pressure as the low-pressure condition develops. Researchers use the observed pressure to keep the process defined and reproducible, whether the immediate goal is drying, degassing, concentration, or sample preparation.
Chemists select this approach when a material must lose solvent or trapped gas under controlled low-pressure conditions. Drying removes solvent from a sample, degassing removes unwanted gas, and concentration reduces solvent content. The same controlled environment can support sample preparation, while lower boiling points help these operations proceed without relying solely on atmospheric-pressure conditions.
Controlled low-pressure treatment allows researchers to examine how reduced pressure affects reactions, materials, and phase changes. Pressure readings document the environmental condition associated with the process, while the resulting behavior can reveal how a system responds when gas density and solvent boiling conditions differ from atmospheric conditions. This links chamber operation to reproducible chemistry experiments.