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Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
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Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

Article DOI: 10.3791/51117 • 10:16 min • January 16th, 2014
January 16th, 2014 •
1, 1, 1, 1, 1, 2, 1,3
1Natural Resource Ecology Laboratory, Colorado State University, 2Soil Plant Nutrient REsearch, USDA-ARS, 3Department of Soil and Crop Sciences, Colorado State University

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Summary
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This method explains how to build and operate a continuous 13C and 15N isotope labeling chamber for uniform or differential plant tissue labeling. Representative results from metabolic and structural labeling of Andropogon gerardii are discussed.

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Continuous 13C Labeling Chamber 15N Labeling Chamber Uniform Labeling Differential Labeling Metabolic Components Structural Components Plant Isotope Labeling Ecosystem Processes CO2 Fluxes Soil Organic Matter Formation Stable Isotope Biomarker Probing Stoichiometric Relationships Biogeochemical Transformations Litter Decomposition Microbial Utilization Carbon Storage
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