Oviduct Microdissection
Oviduct Microdissection - Related Videos
07:44
Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation
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Cited by 1 •
2021
A method for microdissection of the mouse oviduct that allows collection of the individual segments while maintaining RNA integrity is presented. In addition, non-enzymatic oviductal cell dissociation procedure is described. The methods are appropriate for subsequent gene and protein analysis of the functionally different oviductal segments and dissociated oviductal cells.
07:13
Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts
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Cited by 43 •
2011
Culture of normal cells in their three-dimensional context represents an alternative method to study early events required for cellular transformation and tumorigenesis. This method is used to grow normal ovarian and oviductal cells to study early events in ovarian cancer formation.
12:02
Laser Capture Microdissection of Drosophila Peripheral Neurons
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Cited by 25 •
2010
In this video-article we present a method for isolating single or multiple Drosophila da neurons from third instar larvae using the infrared capture (IR) class of Laser Capture Microdissection (LCM). RNA obtained from the isolated neurons can be readily used for downstream applications including qRT-PCR or microarray analyses.
09:56
Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
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2025
The efficiency of the Improved Genome Editing via Oviductal Nucleic Acids Delivery (I-GONAD) method is comparable to traditional microinjection, which requires zygote collection from donor females and transfer to pseudo-pregnant females. This protocol demonstrates its effectiveness by introducing CRISPR/Cas9-induced mutations into the ROSA26 locus on chromosome 6.
04:02
Embryo Transfer Into Rabbit: A Procedure for Laparoscopy-Guided Transfer of Embryos Into Oviduct of Ovulating Recipient Female Rabbit Model
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2025
This video describes the technique of transferring embryos into the oviduct of a recipient female rabbit via Laparoscopy. It allows embryo remodeling in rabbits during its migration through the oviduct, eventually helping in successful implantation. This technique can also be used for stored embryos with good efficiency.
FAQs
Separating defined oviduct regions allows researchers to relate local anatomy to distinct cellular signaling environments. Because each segment retains its epithelial lining, luminal structure, and regional arrangement, analyses can compare how neighboring areas may support different aspects of gamete function, embryo transport, or developmental progression. This regional resolution connects tissue structure with reproductive tract contributions to early development.
Preserving the epithelial lining and luminal structure maintains the tissue features that define each oviduct microenvironment. Damage or loss of these features could make it harder to interpret regional differences in signaling, gamete function, or embryo transport. Intact segments therefore provide a more informative basis for histology, molecular analysis, imaging, and ex vivo culture.
The technique creates separated segments that can be examined individually rather than treating the oviduct as a uniform structure. Researchers can then associate observed cellular, molecular, or developmental features with particular anatomical regions. This comparison helps determine whether a result reflects a localized microenvironment or a broader property of the reproductive tract.
Preparation begins with viewing the tissue under a stereomicroscope and using fine instruments to remove surrounding tissue. The oviduct is then divided into defined regions while preserving the epithelial lining, lumen, and regional organization. The resulting segments can be directed to histology, molecular analysis, imaging, or ex vivo culture, depending on the experimental question.
Dissected segments support several complementary readouts. Histology examines tissue organization, molecular analysis evaluates region-associated molecular features, and imaging visualizes structural or cellular characteristics. Ex vivo culture provides a controlled setting for studying segment behavior or tissue contributions outside the intact tract. Using these approaches together can connect anatomy with signaling and developmental outcomes.
Oviduct microdissection is particularly useful when the research question concerns how reproductive tract regions affect events after fertilization. Separate segments allow investigators to examine relationships among local tissue environments, gamete function, embryo transport, and early developmental progression. The method therefore provides a controlled way to study how anatomy and cellular signaling contribute to development.