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Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts
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Chapters
- 00:05Title
- 01:43Ovarian Dissection and Tissue Isolation
- 03:20Degradation of Alginate and Collection of Cells
- 04:23Fixation of Alginate Encapsulated Organ Cultures for Paraffin Sections
- 04:46Application of the 3D Ovarian Organ Culture System
- 06:29Conclusion
Culture of normal cells in their three-dimensional context represents an alternative method to study early events required for cellular transformation and tumorigenesis. This method is used to grow normal ovarian and oviductal cells to study early events in ovarian cancer formation.
Tags
Alginate HydrogelsThree-dimensional Organ CultureOvariesOviductsOvarian CancerMortality RateCell Type Of OriginOvarian Surface EpitheliumFallopian Tube FimbriaePrimary CultureIn Vitro CultureBiomarkersAnimal ModelsPrevention StrategiesAlginate Hydrogel CulturePlasmid DNASiRNASmall MoleculesScalpel CutsOvulation ModelingOSE ProliferationPlastic Surfaces
