Fungal Its Region Sequencing
Fungal Its Region Sequencing - Related Videos
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Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
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Cited by 22 •
2013
We describe methods for live-cell video microscopy of Candida albicans phagocytosis by macrophages. These methods enable stage-specific analysis of macrophage migration, recognition, engulfment and phagosome maturation and reveal novel aspects of phagocytosis.
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Microbial and Fungal Diversity - Student Protocol
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2019
Examining Microbial Colony Structures ExpandTo begin, collect your allocated culture plates and then remove the Parafilm and lid from one of them. Place the uncovered plate under a dissecting microscope and then observe the individual communities within each quadrant of the plate. As the colonies are identified, fill out the observed colony properties in the appropriate column in the table. Click Here to download Table 1 Then, repeat the observations for each of the other two bacterial species.
05:06
Microbial and Fungal Diversity - Concepts
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2019
Bacteria and fungi are two highly diverse groups of organisms that can have significant beneficial or detrimental impacts on human health. For this reason, it is important to understand and distinguish between individual species of these groups. As you will recall, biological taxonomists group organisms based on their phylogenetic relatedness. The three domains of life, Bacteria, Archaea, and Eukaryota segregate life into three distinct groupings. While all bacteria fall within the domain...
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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2018
We describe a technique for combining flow cytometry and high throughput sequencing to identify late replicating regions of the genome.
04:14
Establishing a Fungal-Bacterial Co-culture for Biofilm Formation
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2026
Source: Miquel Guennoc, C., et al. New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy. J. Vis. Exp. (2017)This video demonstrates the preparation of a fungal culture on treated cellophane membranes to support colony formation and radial growth. It then shows the cultivation and preparation of soil bacterial cells for interaction assays. Finally, it illustrates how bacterial cells adhere to the fungal colony...
FAQs
The ITS region’s variability provides sequence differences that can support taxonomic separation among fungi. After PCR and sequencing, researchers compare the resulting sequences with reference databases to assign organisms to taxonomic groups or identify them when the match is informative. This marker connects DNA variation with measurements of fungal diversity and community composition in environmental samples.
PCR amplification produces copies of the targeted ITS region before sequencing, making this fungal marker available for analysis from a mixed environmental sample. The amplified DNA can then be sequenced and compared with reference databases. This step is especially relevant when samples contain fungi that cannot be cultured, because analysis does not depend on recovering each organism in culture.
Individual identification focuses on assigning a sequence to a fungal taxon, whereas metabarcoding examines the collection of fungal sequences in a mixed environmental sample. ITS sequencing supports both uses because the same marker can be compared with reference databases while the combined results describe community composition. This distinction allows studies of particular fungi or broader assemblages.
A supported workflow moves from an environmental sample to a sequence-based interpretation. Researchers first target the fungal ITS region with PCR, then sequence the amplified DNA, and finally compare those sequences with reference databases. The resulting classifications can describe which fungi occur in a sample and how fungal community composition differs among soil, water, plant, or other habitat samples.
Fungal ITS region sequencing can be applied to soil, water, plants, and other habitats, making it useful for comparing fungal communities across environmental settings. The resulting data support observations of fungal diversity, taxonomic composition, and distribution. Such comparisons can help connect habitats or environmental change with differences in the fungi detected, without requiring every organism to be cultured.
In environmental research, the method provides a way to examine ecological and biological relationships through fungal community data. It can contribute to studies of ecosystem function, environmental change, plant-microbe interactions, and the distribution of potentially beneficial or pathogenic fungi. These applications extend beyond naming species because community composition can be considered alongside habitat and ecological questions.