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Immunology and Infection
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Live-cell Video Microscopy of Fungal Pathogen Phagocytosis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis

Live-cell Video Microscopy of Fungal Pathogen Phagocytosis

DOI:
10.3791/50196-v

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08:52 min

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January 09, 2013

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, , , ,
1Division of Applied Medicine,University of Aberdeen, 2Aberdeen Fungal Group,University of Aberdeen

Chapters

  • 00:05Title
  • 01:44C. albicans Growth and Conditions
  • 02:38C. albicans Staining Using Fluorescein Isothiocyanate (FITC)
  • 03:34Preparation of the J774.1 Mouse Macrophage Cell Line
  • 04:55Live Cell Video Microscopy Phagocytosis Assay 28
  • 06:40Analyzing the Live Cell Video
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

We describe methods for live-cell video microscopy of Candida albicans phagocytosis by macrophages. These methods enable stage-specific analysis of macrophage migration, recognition, engulfment and phagosome maturation and reveal novel aspects of phagocytosis.

Tags

Keywords: Live-cell Video MicroscopyFungal Pathogen PhagocytosisPhagocytic ClearancePattern Recognition ReceptorsPhagosome MaturationInnate Immune Cell FunctionFungal PathogenesisMacrophage UptakeCell CycleVesicular Trafficking

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