Substrate Seeding
Substrate Seeding - Related Videos
02:33
Seed Structure and Early Development of the Sporophyte
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2020
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo. The embryo contains a double set of chromosomes, one set from each parent. Fertilization of the haploid egg by the haploid sperm gives rise to the zygote, which develops into the embryo. The endosperm is a...
04:49
MSC Seeded Scaffold Preparation: A Technique to Prepare Scaffold Seeded with Mesenchymal Stem Cells for Surgical Implantation into Murine Model
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2025
This video describes the technique of seeding mesenchymal stem cells (MSCs) onto a biodegradable poly(lactic acid) (PLA) scaffold. The prepared scaffolds can be implanted into the surgical resection cavity of the brain from where they may migrate towards tumor foci. Further, these cells can be engineered to express therapeutic proteins to stimulate apoptosis in cancer cells.
08:52
A Seed Coat Bedding Assay to Genetically Explore In Vitro How the Endosperm Controls Seed Germination in Arabidopsis thaliana
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Cited by 21 •
2013
We present the procedure to assemble a seed coat bedding assay (SCBA) from Arabidopsis thaliana seeds. The SCBA was shown to be a powerful tool to explore genetically and in vitro how the endosperm controls seed germination in dormant seeds and in response to light cues. The SCBA is in principle applicable under any situation where the endosperm is suspected to influence embryonic growth.
03:42
Introduction to Seed Plants
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2026
Most plants are seed plants—characterized by seeds, pollen, and reduced gametophytes. Seed plants include gymnosperms and angiosperms. Gymnosperms—cycads, ginkgo biloba, gnetophytes, and conifers—typically form cones. The pollen cones contain male gametophytes. The ovulate cones contain female gametophytes and form exposed seeds when fertilized. Angiosperms, the most diverse and ubiquitous group of land plants, form flowers, and fruit. Like the cones of gymnosperms, the flowers and fruit of...
08:09
Preparation and Testing of Plant Seed Meal-based Wood Adhesives
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Cited by 15 •
2015
To facilitate the effort in seeking more economic and environment-friendly formulations of natural product-based wood adhesives, this work demonstrates the preparation and testing of plant seed-based wood adhesives. This protocol allows one to assess plant seed-based agricultural products as suitable candidates for the substitution of synthetic-based wood adhesives.
FAQs
Establishment occurs through a sequence of attachment, acclimation, and growth. First, the introduced organisms remain associated with the material or environmental surface. They then adjust to local conditions before increasing in activity and abundance. Successful establishment depends on whether the surrounding environment supports biological activity, allowing the seeded population to develop into a functioning microbial community.
Moisture, nutrient availability, temperature, and pH are central controls on the process. When these factors fall within conditions that support microbial activity, seeded organisms are more likely to acclimate and grow. If local conditions do not provide adequate support, colonization and the intended biological process may remain limited, reducing the effectiveness of the intervention.
Biofilm formation helps organize attached microorganisms into a developing community on the substrate. This can accelerate biological activity at the treated surface and support the transformation of organic matter or environmental contaminants. As a result, seeding can help establish microbial communities that contribute to improved performance in systems designed for environmental treatment or recovery.
The approach increases the likelihood that organisms capable of useful biological activity will become established where transformation is needed. After attachment and acclimation, their growth can contribute to microbial communities that transform environmental contaminants. Its effectiveness still depends on suitable moisture, nutrients, temperature, and pH, because these conditions determine whether the introduced organisms remain active.
A basic application begins by introducing selected microorganisms or other biological agents onto the target material or environmental surface. The seeded organisms then attach, acclimate to local conditions, and grow when the environment supports their activity. This sequence is intended to initiate colonization, encourage biofilm formation, and establish biological activity suited to the system's purpose.
Environmental applications include wastewater treatment, soil restoration, bioremediation, and engineered ecosystems. In wastewater systems, seeding can help establish communities that improve biological performance. In soils and other degraded environments, it can support recovery by promoting organisms that transform organic matter or contaminants. The specific value depends on matching biological activity with local environmental conditions.