Bibac-gw Vector
Bibac-gw Vector - Related Videos
09:20
Vectors in Multiple Directions
0 Views •
2023
Source: Nicholas Timmons, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA This experiment demonstrates how vectors add and subtract in multiple directions. The goal will be to analytically calculate the addition or subtraction of multiple vectors and then to experimentally confirm the calculations. A vector is an object with both magnitude and direction. The magnitude of a vector is simply denoted as the length,...
10:42
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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Cited by 21 •
2017
We describe the production strategy of integrase-deficient lentiviral vectors (IDLVs) as vehicles for delivering CRISPR/Cas9 to cells. With an ability to mediate quick and robust gene editing in cells, IDLVs present a safer and equally effective vector platform for gene delivery compared to integrase-competent vectors.
01:30
Vectors
0 Views •
2025
Vectors are mathematical entities characterized by both magnitude and direction. Unlike scalars, which are defined solely by magnitude, vectors represent quantities like displacement, velocity, and force, where direction is essential. Vectors are graphically represented as directed line segments, extending from an initial point to a terminal point, denoted with bold letters or arrows placed above the symbol. Two vectors are deemed equal if they share identical magnitudes and directions,...
03:20
Large-Scale Production of High-Capacity Adenoviral Vectors
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2026
Source: Ehrke-Schulz, E., et al. Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5. J. Vis. Exp. (2016)This video demonstrates the large-scale amplification of high-capacity adenoviral vectors in suspension-adapted human embryonic kidney cells expressing Cre recombinase for use in gene delivery applications requiring high-titer, helper-free vector preparations.
08:47
Intracranial Injection of Adeno-associated Viral Vectors
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Cited by 56 •
2010
Here we present the intracranial injection of AAV vectors for fluorescent labeling of neurons and glia in the visual cortex.
FAQs
BAC-based replication and maintenance let the vector preserve genomic DNA fragments that exceed the practical capacity of many conventional plasmids. This is important when an experiment must retain a complex locus rather than only a short coding sequence. The bacterial phase therefore supports propagation of large inserts before they are used for plant genetic analysis.
Gateway recombination sites separate the initial cloning step from placement in the final destination vector. An insert can first be held in an entry clone and then transferred efficiently into BiBAC-gw through recombination. This arrangement simplifies movement of a prepared genomic fragment into a vector configured for subsequent delivery into plant cells.
T-DNA border sequences provide the boundaries associated with transfer of the cloned region during Agrobacterium-mediated transformation. After the construct reaches plant cells, these sequences support delivery of the cloned DNA region for integration into the plant genome. Their role connects bacterial vector handling with the plant-cell stage required for genomic functional studies.
A typical workflow begins by placing the genomic fragment in an entry clone, followed by Gateway recombination into the BiBAC-gw destination vector. The recombinant BAC is maintained in bacteria, then used with Agrobacterium to deliver the T-DNA-bounded region into plant cells. This sequence distinguishes construct assembly, bacterial propagation, and plant transformation.
The vector is particularly informative for complex loci whose function may depend on more than a single coding sequence. Regulatory regions and gene clusters can be carried with the genomic fragment, allowing their effects to be examined in plant genetic studies. This broader representation is useful when analyzing locus behavior requires DNA context beyond an isolated gene.
BiBAC-gw supports functional analysis by linking large-fragment cloning to delivery into plant cells and genomic integration. Investigators can therefore examine complex genomic material in a plant context rather than limiting analysis to bacterial maintenance alone. Its value is especially relevant when regulatory DNA or clustered genes are central to the research question and conventional plasmids cannot represent the locus adequately.