Fat Content Measurement
Fat Content Measurement - Related Videos
07:59
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
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Cited by 10 •
2017
This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.
07:38
Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
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Cited by 24 •
2015
Milk is a primary source of nutrition for the neonate. Analysis of milk components may provide insight into maternal factors that affect offspring health. This protocol describes a manual method of collecting milk samples from the lactating rat, which can then be used for further downstream analysis.
01:23
Measurement of Air Content in Concrete
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2024
Air content measurement in concrete is critical for ensuring structural integrity and durability of concrete structures, especially in environments prone to severe weather conditions. Accurate air content analysis optimizes concrete's resistance to freeze-thaw cycles and enhances its workability and strength. Several methods are standardized under ASTM guidelines to measure the air content in fresh concrete, each suitable for different concrete types and conditions. The pressure method,...
13:09
Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography
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Cited by 26 •
2012
Fat content analysis is routinely conducted in studies utilizing murine obesity models. Emerging methods in small animal CT imaging and analysis are providing for longitudinal detail rich fat content analysis. Here we detail step by step procedures for performing small animal CT imaging, analysis, and visualization.
06:41
Decellularizing Mammary Fat Pads: Deriving Extracellular Matrix Hydrogels from Mammary Fat Pads
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2023
This video describes a technique of deriving extracellular matrix hydrogels by decellularizing murine mammary fat pads following ex vivo irradiation for in vitro studies. This hydrogel helps to mimic the in vivo breast tissue environment post-radiation treatment to study tumor cell behavior.
FAQs
Extraction moves lipids away from other sample components so they can be assessed separately. The workflow may then use the recovered lipid fraction for mass determination, chemical analysis, or instrument-based measurement. This separation is important because the resulting value is intended to represent lipid content rather than an undifferentiated mixture of biological, food, or tissue constituents.
These approaches differ in how the lipid fraction is quantified. Gravimetric analysis determines lipid content from measured mass, whereas chemical methods use a lipid-related chemical measurement. Instrument-based methods determine concentration through an analytical instrument. Selecting among them depends on the sample and the type of result needed for comparison, such as mass or concentration.
Sample type influences how lipids must be separated from other components before quantification. Biological samples, foods, and tissues may therefore require different analytical handling, even when the goal is comparable lipid data. Matching the measurement approach to the sample helps researchers characterize fat-related traits consistently across individuals, strains, or experimental groups.
When the same lipid-related measurement is collected across individuals or strains, differences in fat levels can be treated as phenotypic variation. Genetic studies can then compare those measurements with genetic differences to investigate links involving lipid storage and metabolism. The resulting phenotype data support analysis of inherited traits without treating fat content as a purely environmental characteristic.
A general workflow begins with the biological, food, or tissue sample, followed by lipid extraction when separation is needed. Researchers isolate the lipid fraction from other components and quantify it by mass, chemical analysis, or an instrument-based method. The selected output can then be recorded as a comparable measurement for samples, individuals, or strains.
The measurement is useful when researchers need a quantitative phenotype for studying inherited differences in lipid-related biology. Applications described for this context include evaluating variation among individuals or strains, examining lipid storage and metabolism, supporting animal breeding studies, and contributing to functional genomics. The data also connect genetic analysis with nutritional biology and metabolic disease research.