Whitefly Fertilization Rate

Whitefly fertilization rate is the proportion of eggs fertilized after mating, a reproductive measure that helps explain sex-ratio patterns and genetic variation in whitefly populations. Whiteflies typically exhibit haplodiploid reproduction: fertilized eggs develop into females, whereas unfertilized eggs develop into males, so fertilization depends on successful mating, sperm transfer, and the female’s use of stored sperm. Measuring this rate can support studies of inheritance, population genetics, reproductive compatibility, and biological control, where changes in offspring sex ratios may influence population growth and the effectiveness of parasitoids or other management strategies.

Whitefly Fertilization Rate - Related Videos

Research

JoVE Journal - Biology
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Transmitting Plant Viruses Using Whiteflies

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Cited by 41 •

2013

Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...

Research

JoVE Journal - Biology

Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci

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Cited by 4 •

2017

Here, we present a protocol to isolate endosymbionts from the whitefly Bemisia tabaci through dissection and filtration. After amplification, the DNA samples are suitable for subsequent sequencing and study of the mutualism between endosymbionts and whitefly.

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

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Cited by 4 •

2019

We present a simple cytogenetic technique using 4′,6-diamidino-2-phenylindole (DAPI) to determine the fertilization rate and primary sex ratio of the haplodiploid invasive pest Bemisia tabaci.

Education

JoVE Core - Biology

Fertilization

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2025

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...

Research

JoVE Journal - Biology
Free Sample

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

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Cited by 29 •

2009

This is a high-throughput sperm cryopreservation protocol for zebrafish. Sperm cryopreserved using this protocol has an average of 25% fertility in subsequent vitro fertilization and is stable over many years.

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