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Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique
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JoVE Journal Genetics
Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

DOI:
10.3791/59213-v

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05:24 min

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April 01, 2019

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1Graduate Interdisciplinary Program in Entomology and Insect Science,University of Arizona, 2Department of Entomology,University of Arizona

Chapters

  • 00:04Title
  • 00:31Initial Setup and Dechorionation
  • 02:11Fixation, Staining, Washing, and Mounting
  • 03:49Results: Influence of Rickettsia sp. nr. bellii Endosymbiont on Primary Sex Ratio of B. tabaci
  • 04:51Conclusion

Summary

Automatic Translation

Automatic Translation

We present a simple cytogenetic technique using 4′,6-diamidino-2-phenylindole (DAPI) to determine the fertilization rate and primary sex ratio of the haplodiploid invasive pest Bemisia tabaci.

Tags

Keywords: Bemisia TabaciWhiteflyCytogenetic TechniqueEgg Fertilization RateHaplodiploidSex RatioOvipositionDechorionationDAPI StainingMicroscopy

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