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Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

Article DOI: 10.3791/59213-v • 05:36 min • April 1st, 2019
April 1st, 2019 •
1, 2
1Graduate Interdisciplinary Program in Entomology and Insect Science, University of Arizona, 2Department of Entomology, University of Arizona

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Summary
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We present a simple cytogenetic technique using 4′,6-diamidino-2-phenylindole (DAPI) to determine the fertilization rate and primary sex ratio of the haplodiploid invasive pest Bemisia tabaci.

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Egg Fertilization Rate Bemisia Tabaci Cytogenetic Technique Whitefly Behavior And Ecology Haplodiploid Oviposition Sex Ratio Protocol Petri Dish Humidity Chamber Probe Glass Pipette Tips Whitefly Eggs Microscope Slide Paraffin Film Dechorionation Sodium Hypochlorite Bleach Solution Glacial Acetic Acid DAPI
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