Collagen Second Harmonic
Collagen Second Harmonic - Related Videos
09:23
Harmonic Nanoparticles for Regenerative Research
0 Views •
Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
04:50
Reproducibility and Harmonization in Research Using Biological Standards: The Example of Platelet Agonist Collagen-Related Peptide
0 Views •
2023
Here we present a method to standardize the platelet agonist collagen-related peptide cross-linked (CRP-XL) using light transmission aggregometry. While the protocol is targeted at platelet function, the experimental process can be applied to most biological molecules and bioassays to ensure scientific rigor and reproducibility.
09:19
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
0 Views •
Cited by 3 •
2025
This protocol presents a step-by-step guide for the Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopic technique, including details on how to generate the laser light source, prepare a tissue sample, conduct imaging, and analyze the data. SLAM advances nonlinear microscopy by measuring four complementary label-free contrasts to investigate the tissue microenvironment.
01:09
Harmonic Mean
0 Views •
2023
The arithmetic mean is usually skewed towards the larger values in the data set. Therefore, to avoid this inherent bias towards smaller values, the harmonic mean is used. Take the example of the speed of a car, which is the measure of the rate of distance traveled. If the vehicle traverses the same distance back-and-forth, its average speed equals the total distance traveled divided by the total time taken. However, if the car moves with varying speeds, then the arithmetic mean is more skewed...
07:52
Hooke's Law and Simple Harmonic Motion
0 Views •
2023
Source: Ketron Mitchell-Wynne, PhD, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA Potential energy is an important concept in physics. Potential energy is the energy associated with forces that depend upon the position of an object relative to its surroundings. Gravitational potential energy, which is discussed in another video, is the energy associated that is directly proportional to the height of an object above...
FAQs
The signal depends on the ordered, noncentrosymmetric arrangement of collagen fibrils. This organization allows two excitation photons to interact with the tissue and produce one emitted photon at twice the frequency. Consequently, the measurement is sensitive to fibril arrangement and collagen architecture, not simply to whether collagen is present in the tissue.
Because the signal originates from the tissue’s own ordered collagen arrangement, imaging can reveal extracellular matrix structure without adding a label for collagen. This feature supports visualization of matrix organization during tissue injury, fibrosis, immune responses, or pathogen-associated changes, allowing structural observations to remain connected to the native tissue context.
Matrix remodeling, tissue injury, fibrosis, and changes in collagen architecture can alter the structural pattern revealed by the signal. Interpreting those patterns therefore focuses on how the extracellular matrix is organized and changing. Researchers can use that information to examine relationships among matrix structure, inflammation, disease progression, and tissue repair.
SHG microscopy follows collagen architecture as tissue changes during an immune response, allowing investigators to visualize matrix remodeling and injury as they occur. These observations connect evolving extracellular matrix structure with inflammation and tissue repair, providing a tissue-level view of how immune-associated changes affect the organization of collagen in biological tissues.
During pathogen invasion, SHG microscopy can reveal changes in collagen architecture and associated tissue injury through the collagen-derived signal. Researchers can relate these structural changes to extracellular matrix remodeling, inflammation, and disease progression. This places infection-associated tissue damage in its matrix context and helps connect pathogen invasion with changes in tissue organization.
Fibrosis involves changes in the extracellular matrix, and SHG makes collagen organization visible as those changes develop. Examining matrix architecture allows investigators to relate collagen remodeling to tissue injury, inflammatory processes, and the broader course of disease. The resulting structural information helps characterize fibrosis through its effects on the organization of collagen.
By enabling real-time visualization of collagen architecture, the method can track matrix changes during repair after injury. Researchers can compare these structural changes with inflammation and disease progression, using extracellular matrix organization as a link between the tissue response and the developing repair process. This helps place repair within the broader sequence of tissue remodeling.