Inverted Light Microscopy
Inverted Light Microscopy - Related Videos
08:35
Introduction to Light Microscopy
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2023
The light microscope is an instrument used by researchers in many different fields to magnify specimens to as much as a thousand times their original size. In its simplest form, it is composed of a clear lens that magnifies the sample and a light source to illuminate it. However, most light microscopes are much more complex and house numerous fine-tuned lenses with tightly controlled dimensions all within the body of the microscope itself and in components such as the objectives and eyepieces.
06:13
The Inverted-face Effect
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University In perception, it is often the case that the ability to recognize and interpret complex stimuli feels effortless but actually demands complicated and intensive processing. This is because processing is specialized and automated for certain types of very important stimuli. Among the best examples of this phenomenon is face processing. People do not try to detect and recognize faces. It just seems to happen. However, detecting faces...
10:00
Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
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Cited by 11 •
2011
We demonstrate the fabrication of a low-cost cryogenic stage designed to fit most reflected light microscopes. This lab-built cryogenic stage enables efficient and reliable correlative imaging between cryo-light and cryo-electron microscopy.
11:16
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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Cited by 12 •
2016
A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.
01:20
Inverting and Non-inverting OpAmps
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2024
In an inverting amplifier, the input voltage is connected through a resistor to the inverting terminal. Meanwhile, the non-inverting terminal is grounded and a feedback resistor is established between the inverting and output terminal, as depicted in Figure 1. Figure 1: The inverting amplifier The objective is to discern the relationship between the input voltage (vi) and the output voltage (vo). By applying Kirchhoff's Current Law (KCL) and presuming the operational amplifier to be ideal, the...
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The lower objective allows the specimen to remain in its culture vessel while the imaging system collects light from beneath. This arrangement limits the need to remove or reposition the sample during observation, which is especially relevant when researchers want to follow living immune cells or infection-associated changes without interrupting the culture. It therefore supports time-resolved examination of cellular behavior.
Its geometry accommodates specimens held in containers and allows light to pass through the sample from above or through the vessel base before collection below. That compatibility makes the approach practical for cell-culture experiments in which preserving the original vessel and observing the same specimen repeatedly are important. In immunology, this supports longitudinal study of cellular behavior during changing experimental conditions.
The optical path can support collection of either transmitted light or an emitted image from the specimen. This gives investigators a way to examine cellular material under the imaging mode available in the experiment while retaining the lower-objective configuration. In infection studies, the resulting observations can be used to assess morphology, host-pathogen interactions, or changes associated with infection.
Repeated imaging creates a time-based record rather than a single snapshot. Researchers can use that record to follow immune-cell migration, changes in morphology, and infection-associated alterations as an experiment progresses. Because the same culture can be observed over time, the method helps relate cellular behavior to the course of an experimental response, supporting quantitative assessment when measurements are collected consistently.
A basic workflow places the sample in a culture vessel, directs light through the sample from above or through the vessel base, and positions the objective below to collect the transmitted or emitted image. Investigators can then repeat observations of the culture to examine behavior, morphology, or responses over time. The procedure is therefore compatible with longitudinal cell-based experiments.
In immunology and infection research, this approach is useful when the question depends on observing cells and their interactions in culture. Supported applications include monitoring living immune cells, examining host-pathogen interactions, tracking cell migration, and assessing infection-associated changes. These uses connect optical observations with dynamic cellular behavior rather than relying only on a final endpoint.
The resulting images can support both descriptive and quantitative evaluation. Researchers may examine cellular morphology, follow changes in cellular behavior, and assess responses to an experimental condition, including infection-associated changes. When observations are repeated, comparisons across time can show how the culture changes during the experiment. This makes the method valuable for linking observable cellular changes with immunological or infectious processes.