Luminal Pressure
Luminal Pressure - Related Videos
07:26
Synthesis and Microdiffraction at Extreme Pressures and Temperatures
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Cited by 5 •
2013
The laser heated diamond anvil cell combined with synchrotron micro-diffraction techniques allows researchers to explore the nature and properties of new phases of matter at extreme pressure and temperature (PT) conditions. Heterogeneous samples can be characterized in situ under high pressure by 2D mapping and combined powder, single-crystal and multigrain diffraction approaches.
08:24
Profiling Luminal pH in Three-Dimensional Gastrointestinal Organoids Using Microelectrodes
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Cited by 2 •
2024
The present protocol describes pH measurements in human tissue-derived gastric organoids using microelectrodes for spatiotemporal characterization of intraluminal physiology.
10:21
Studying Murine Small Bowel Mechanosensing of Luminal Particulates
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Cited by 3 •
2022
To study how the small bowel handles particulates of varying sizes, we have modified an established in vivo method to determine small bowel transit.
09:59
Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
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Cited by 8 •
2017
This protocol describes methods for purifying, quantitating, and characterizing extracellular vesicles (EVs)/exosomes from non-adherent/mesenchymal mammary epithelial cells and for using them to transfer mammary gland-forming ability to luminal mammary epithelial cells. EVs/exosomes derived from stem-like mammary epithelial cells can transfer this cell property to cells that ingest the EVs/exosomes.
07:25
Assessing Murine Resistance Artery Function Using Pressure Myography
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Cited by 18 •
2013
In pressure myography, an intact small segment of a vessel is mounted onto two small cannulas and pressurized to a suitable luminal pressure. Here, we describe the method to measure vasorelaxation response of the mouse 3rd order mesenteric arteries in c57 and sGCα1-/- mice using pressure myography.
FAQs
Changes in pressure can stretch epithelial and immune cells, activating mechanosensitive signaling pathways. These signals may alter barrier function, which is important because epithelial barriers regulate interactions between tissue, luminal contents, and microorganisms. Studying this response helps researchers connect physical distension with changes in host defense and inflammatory activity.
Pressure changes reflect shifts in the amount and movement of material inside a hollow structure. Volume changes, secretion, absorption, motility, and flow can each modify the local mechanical environment. Examining these factors together helps distinguish whether altered pressure arises from accumulated contents, disrupted transport, or changes in the normal movement of material.
Altered pressure can influence how microbes move through a tissue lumen and may contribute to impaired drainage. These mechanical changes can affect microbial persistence and the likelihood of translocation across epithelial barriers. Consequently, pressure analysis adds a physical dimension to studies of inflammation, pathogen survival, and progression of infection.
Researchers investigate it by measuring or experimentally manipulating pressure within relevant hollow biological structures. They can then examine relationships with tissue distension, epithelial barrier function, inflammation, microbial movement, and translocation. This approach helps determine whether mechanical changes accompany disease processes or contribute to altered host-pathogen interactions.
Both structures contain luminal contents and epithelial barriers that can respond to changes in internal mechanical conditions. In these settings, pressure-related distension, altered flow, or impaired drainage may affect microbial distribution and tissue responses. Comparing such sites helps place mechanical influences within broader studies of mucosal host defense and infection.
Pressure-focused studies can identify how abnormal tissue mechanics relate to inflammation, pathogen persistence, and movement across epithelial barriers. These findings may guide strategies aimed at restoring more normal mechanical conditions rather than addressing immune or microbial factors alone. The resulting perspective supports investigation of disease progression and treatments that improve tissue mechanics.