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Studying Murine Small Bowel Mechanosensing of Luminal Particulates
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JoVE Journal Medicine
Studying Murine Small Bowel Mechanosensing of Luminal Particulates

Studying Murine Small Bowel Mechanosensing of Luminal Particulates

DOI:
10.3791/63697-v

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10:21 min

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March 18, 2022

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, , , ,
1Enteric NeuroScience Program (ENSP), Division of Gastroenterology & Hepatology, Department of Medicine,Mayo Clinic, 2Medical Scientist Training Program (MSTP),Mayo Clinic, 3Easy Learning Labs, 4Department of Physiology & Biomedical Engineering (PBME),Mayo Clinic

Chapters

  • 00:05Introduction
  • 00:49Intraluminal Content Gavage and Bowel Dissection
  • 03:42Ex Vivo Imaging and Analysis
  • 07:47Results: Fluorescence Traces Analysis, Geometric Center of Fluorescence Distribution, and Categorization of Power Spectra
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

To study how the small bowel handles particulates of varying sizes, we have modified an established in vivo method to determine small bowel transit.

Tags

Keywords: Small IntestineMechanosensingLuminal ParticulatesGut MechanosensitivityGavageIn Vivo ImagingIntestinal TransitMouse ModelRITC SolutionMicrospheres

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