Mouse Model Establishment
Mouse Model Establishment - Related Videos
08:40
Establishment and Characterization of UTI and CAUTI in a Mouse Model
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Cited by 51 •
2015
The ability to model urinary tract infections (UTI) is crucial in order to be able to understand bacterial pathogenesis and spawn the development of novel therapeutics. This work’s goal is to demonstrate mouse models of experimental UTI and catheter associated UTI that recapitulate and predict findings seen in humans.
03:42
Establishing a Mouse Model of Vaginal Group B Streptococcus Infection
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2026
Source: Patras, K. A., Doran, K. S. A Murine Model of Group B Streptococcus Vaginal Colonization. J. Vis. Exp. (2016)This video demonstrates the preparation and vaginal inoculation of a hormone-treated female mouse with Group B Streptococcus to model infection. It explains how to culture and prepare the bacteria, administer the inoculum into the vaginal lumen, and ensure successful infection.
02:26
Establishing a Mouse Model of Small Fiber Neuropathy Using Resiniferatoxin
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2025
This video demonstrates a method for establishing a small fiber neuropathy model in mice using the TRPV1 agonist resiniferatoxin (RTX). It outlines the steps involved in administering RTX to mice and explains how RTX induces neuronal death in small-diameter sensory neurons, thereby establishing the neuropathy model.
02:47
Establishing a Mouse Model of Burn Infections with Pathogenic Bacteria
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2026
Source: Wang, Y., et al. In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging. J. Vis. Exp. (2017)This video demonstrates how to establish a burn infection in a mouse model by applying a heated brass block followed by pathogenic bacterial inoculum. The bacteria colonize the exposed extracellular matrix and form a biofilm, enabling in vivo studies of host-pathogen interactions.
03:05
Establishing a Focal Demyelination Mouse Model via a Two-Point Injection of Lysophosphatidylcholine
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2025
This video demonstrates the generation of a focal demyelination mouse model using a two-point Lysophosphatidylcholine (LPC) injection. The skull of an anesthetized mouse is exposed. The injection coordinates are determined, and a hole is drilled to enable LPC injections at targeted sites. LPC induces focal demyelination of neurons via inflammation, while a two-point injection ensures its sustainability.
FAQs
Mouse strain is a central experimental variable because it can affect the immune response, infection-related findings, and disease features reproduced in the system. Researchers should select a strain that matches the biological question and define the relevant experimental conditions consistently. Careful selection improves interpretation by reducing variation that could otherwise obscure host-pathogen interactions or treatment effects.
Several approaches can create distinct experimental conditions, including genetic modification, pathogen inoculation, transplantation, and controlled immune manipulation. Each approach emphasizes different biological features, so the choice should follow the question being tested. For example, researchers may focus on host responses, pathogen burden, inflammation, or tissue changes, depending on how the model is established and monitored.
Controls and defined conditions distinguish effects caused by the experimental intervention from normal variation or unrelated changes. Consistent strain selection, treatment conditions, monitoring, and comparison groups support reproducibility and make immune activity, pathogen burden, and tissue findings easier to interpret. Without this structure, apparent differences may not reliably reflect the mechanism or disease feature under investigation.
A useful model should produce measurable findings linked to the research question, such as clinical signs, pathogen burden, immune-cell activity, or tissue changes. Researchers evaluate these outcomes together rather than relying on a single measurement. Concordant findings can clarify host-pathogen interactions, inflammation, or protection, while inconsistent results may indicate that the selected conditions do not reproduce the intended feature adequately.
A typical workflow begins by selecting the mouse strain and defining the target immune, infection, or disease features. Researchers then choose an appropriate approach, such as pathogen inoculation, genetic modification, transplantation, or immune manipulation, and establish controls and monitoring conditions. Clinical signs, pathogen burden, immune-cell activity, and tissue changes are assessed to validate the resulting system.
These models are used before clinical studies when researchers need to examine protection, pathogen control, inflammation, or immune activity under controlled conditions. Vaccine studies can assess protective responses, while antimicrobial and immunotherapy studies can examine changes in pathogen burden or host responses. The model must reproduce the relevant biological feature well enough for treatment-related outcomes to be interpreted.
In immunology and infection research, carefully designed mouse systems help connect experimental interventions with host-pathogen interactions, inflammatory mechanisms, and protective responses. Researchers can examine clinical signs alongside pathogen burden, immune-cell activity, and tissue changes. Humane endpoints remain essential throughout the study, supporting responsible experimentation while helping preserve reliable and reproducible observations.