Establishment and Characterization of UTI and CAUTI in a Mouse Model

The overall goal of this procedure is to display a reliable method for the establishment of urinary tract infections or UTIs and catheter associated UTIs, or ca a UTI I in a mouse model, and to assess the infections. This is accomplished by first properly preparing the bacterial inoculums, the catheter and needle. Next, the mouse urinary tract is intravesical, inoculated, and catheterized.

Then the urinary tract tissues and catheters are harvested to evaluate the infection. Finally, the infection is quantified by determining bacterial tissue, urine, and catheter load and or intracellular bacterial community or IBC formation. Ultimately, CFU enumeration and laxy staining are used to show the establishment persistence and severity of urinary tract colonization within a mouse model.