Murine Leukemia Virus Core
Murine Leukemia Virus Core - Related Videos
08:31
Murine Model of Leukemia Relapse to Induction Chemotherapy for Acute Lymphoblastic Leukemia
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2025
The protocol aims to describe a mouse model for generating relapses of acute lymphoblastic leukemia, based on the dynamics of the response to induction chemotherapy.
08:00
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
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Cited by 29 •
2016
This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining.
02:42
In Vitro Disassembly of the Influenza A Virus Core Using pH-Controlled Gradient Centrifugation
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2026
Source: Stauffer, S. et al. In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation. J. Vis. Exp. (2016)This video demonstrates the pH-dependent disassembly of influenza A virus (IAV) cores using gradient ultracentrifugation. It outlines glycerol gradient preparation, ultracentrifugation, and how different pH conditions affect IAV core disassembly.
02:10
The Nucleosome Core Particle
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2020
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins. The paradox Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
02:36
Establishing a Murine Tumor Cell Line In Vitro Using a Virus-Cas9 System
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2026
Source: Prasad, M. et al. In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System. J. Vis. Exp. (2020)This video demonstrates the in vitro transformation of murine epithelial cells through viral delivery of Cre recombinase and mutation-inducing components. It outlines the steps for Cas9 activation, cancer-gene modification, and the establishment of a tumorigenic murine cell line.
FAQs
The RNA genome, nucleocapsid proteins, reverse transcriptase, and integrase contribute to a linked delivery process. Nucleocapsid proteins associate with the genome, while reverse transcriptase supports conversion of viral RNA into DNA after entry. Integrase then supports insertion of that DNA into the host genome. Studying these coordinated roles helps explain how the core connects entry with replication.
Core disassembly makes the internal genome and viral enzymes available at the appropriate stage after the virus enters a host cell. If the core remained intact, the RNA could not be copied into DNA and the relevant components could not participate in integration. Examining this transition therefore helps clarify how retroviral entry progresses into genome replication and persistence.
Reverse transcriptase and integrase act at different stages of the retroviral replication process. Reverse transcriptase enables the viral RNA genome to be copied into DNA, whereas integrase supports incorporation of that DNA into the host genome. Considering these enzymes together shows why the core is more than a protective structure: it carries functions needed to connect incoming viral material with host-cell genetic information.
The MLV core provides a defined viral structure and contains viral RNA and proteins that can be examined in studies of host immune recognition. Investigators can use the core to ask how infected cells or immune defenses respond to retroviral components, while also relating recognition to core assembly, entry, and disassembly. This connects structural virology with immunology and infection research.
Research on MLV core assembly and entry can reveal how retroviral components are organized before infection and how they become available after host-cell entry. These studies help distinguish events associated with assembly, delivery, uncoating, and replication. The resulting information supports broader analysis of retroviral infection mechanisms and can guide work involving MLV-based viral vectors.
The MLV core is relevant to viral-vector research because it illustrates how a retroviral system packages nucleic acid with proteins and enzymes, delivers that material into a host cell, and supports DNA integration. Studying these properties helps researchers understand the biological basis of vector behavior. It also provides context for evaluating how retroviral components are used in infection and gene-delivery studies.