Non-surgical Method
Non-surgical Method - Related Videos
09:26
Method for Novel Anti-Cancer Drug Development using Tumor Explants of Surgical Specimens
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Cited by 8 •
2011
Here, we established a method for drug efficacy testing with surgical specimens of brain tumors, termed “tumor explant method”. With this method, we can evaluate drug efficacy without breaking the microenvironment of solid tumors. To validate reliability of this method, we describe representative data with our glioma specimen treated with the current first-line chemotherapeutic agent, temozolomide.
08:01
Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease
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Cited by 9 •
2016
This article includes detailed protocols for genetic labeling of mouse skin, surgical denervation, skin biopsy and visualizing labeled epithelia by whole-mount β-galactosidase staining. These methods can be used to test the requirement for nerves in mouse models of normal and pathological skin.
04:10
Surgical Cisterna Magna Injection: A Method for Administering Tumor Cells Directly into Central Nervous System of Murine Model
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2023
In this video, we describe the procedure to deliver human-derived tumor cells into the cisterna magna of a mouse’s brain. The orthotopic mouse model can be used to study metastatic spread of cancer to the cranial meninges.
05:05
Locating the Cisterna Magna in a Pig Model: A Surgical Method to Access the Cisterna Magna in a Pig Model for Direct Cannulation
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2025
In this video, we describe a method to access the cisterna magna in a pig model. The cisterna magna is a preferred site for studies concerning cerebrospinal fluid.
03:59
Surgical Correction for Pediatric Epiblepharon and Trichiasis
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2025
Here, we present a protocol for surgical correction for pediatric epiblepharon and trichiasis.
FAQs
They examine different biological signals according to the question being asked. Immune markers indicate host responses, whereas pathogen components provide evidence of the infectious agent itself. Measuring these signals in collected fluids can help separate immune activation from direct detection of microbial material, supporting screening, infection surveillance, and decisions about further evaluation.
Collected biological fluids provide measurable material without requiring tissue disruption. Researchers can analyze immune markers or pathogen components in these samples and compare results across time. This approach makes it possible to monitor changing immune function, evaluate infection status, and obtain clinically useful information while reducing the burden associated with invasive tissue collection.
Targeted agents can influence either the microorganism or the host response. Medicines may alter microbial growth, while other agents regulate immune activity. These distinct effects allow investigators and clinicians to study treatment response from complementary perspectives: changes in infection-related signals may reflect microbial control, while changes in immune markers may indicate altered host function.
Repeated measurements allow the same individual to be followed over time rather than assessed at only one moment. Trends in immune markers, pathogen components, or treatment-related responses can reveal progression, persistence, or improvement. This longitudinal perspective supports monitoring and can provide a clearer basis for evaluating infection status and immune function.
A basic workflow begins by selecting the relevant biological signal, such as an immune marker or pathogen component, and obtaining an appropriate collected fluid. The signal is then measured and interpreted in relation to screening, surveillance, or treatment assessment. Repeating the same measurement can extend the workflow into longitudinal monitoring of the individual.
They are particularly useful when researchers need screening, infection surveillance, treatment selection, or repeated assessment of immune function. Their lower procedural burden can support measurements across multiple time points and may improve access to care. In infection studies, the resulting data can connect pathogen detection, host responses, and medicine-related changes without relying on operative tissue sampling.