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Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease
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Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease

Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease

DOI:
10.3791/54050-v

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08:01 min

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June 26, 2016

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, ,
1Dermatology, Cell and Developmental Biology,University of Michigan, 2Dermatology Branch, National Cancer Institute,National Institutes of Health

Chapters

  • 00:05Title
  • 00:43Collecting Skin Biopsies
  • 02:49Surgical Denervation
  • 05:36Behavioral Test of Denervation
  • 06:22Results: Touch Dome Cells and Merkel Cells Require Innervation
  • 07:20Conclusion

Summary

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Automatic Translation

This article includes detailed protocols for genetic labeling of mouse skin, surgical denervation, skin biopsy and visualizing labeled epithelia by whole-mount β-galactosidase staining. These methods can be used to test the requirement for nerves in mouse models of normal and pathological skin.

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Cutaneous Surgical DenervationMouse Skin Disease ModelsNerve Influence On SkinSkin BiopsySkin Sample PreparationHistology And Whole-mount Staining

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