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Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease
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Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease

Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease

English

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9,600 Views

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08:01 min

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June 26, 2016

DOI:

10.3791/54050-v

DOI:
10.3791/54050-v

•

08:01 min
June 26, 2016

•

11 Views
, ,
1Dermatology, Cell and Developmental Biology,University of Michigan, 2Dermatology Branch, National Cancer Institute,National Institutes of Health

Summary

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This article includes detailed protocols for genetic labeling of mouse skin, surgical denervation, skin biopsy and visualizing labeled epithelia by whole-mount β-galactosidase staining. These methods can be used to test the requirement for nerves in mouse models of normal and pathological skin.

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Cite this Article

Peterson, S. C., Brownell, I., Wong, S. Y. Cutaneous Surgical Denervation: A Method for Testing the Requirement for Nerves in Mouse Models of Skin Disease. J. Vis. Exp. (112), e54050, doi:10.3791/54050 (2016).

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