Peritoneal Fluid Harvesting
Peritoneal Fluid Harvesting - Related Videos
04:59
Peritoneal Low-density Neutrophil Isolation: A Technique to Obtain Low-density Neutrophils from Peritoneal Lavage Fluid Using Magnetic Activated Cell Sorting
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2023
In this video, we describe the procedure for isolating low-density neutrophils, or LDNs, from the peritoneal lavage fluid of a postoperative human patient. The LDNs thus obtained can be used in further downstream experiments to understand their role in tumor growth and progression.
03:41
Harvesting Peritoneal Fat Depots: A Technique to Study Ovarian Cancer Colonization of Peritoneal Adipose Tissues in Mouse Models
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2023
In this video, we use a euthanized female mouse model bearing ovarian cancer. We then describe a protocol to identify and extract fat depots or adipose tissues associated with the five primary peritoneal organs to study metastatic cancer cell colonization.
04:55
Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid
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2025
This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.
04:32
Isolation of Mouse Peritoneal Cavity Cells
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Cited by 445 •
2010
The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.
05:05
In Vivo Activation of Peritoneal Macrophages in Response to Antibodies in a Murine Model
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2025
This video demonstrates the activation of a mouse's peritoneal macrophages using intravenous immunoglobulin and bacterial lipopolysaccharide. The co-stimulation activates the peritoneal macrophages into anti-inflammatory macrophages possessing immune regulatory activity.
FAQs
Lavage increases the amount of recoverable material when direct aspiration yields little fluid. Introducing sterile fluid into the peritoneal cavity and then recovering it helps sample resident leukocytes, soluble inflammatory mediators, and possible infectious agents from the same localized environment. This makes lavage particularly useful for comparing immune or infectious changes across experimental conditions.
Resident leukocytes provide cellular evidence of the local immune response, while soluble mediators indicate inflammatory signaling within the cavity. Infectious agents, when present, provide information about pathogen involvement. Examining these components together allows investigators to relate cellular responses to mediator changes and pathogen presence rather than relying on a single type of measurement.
Sampling changes over time can show how local immune cells, soluble mediators, or infectious agents vary during an experiment. Comparing these patterns helps investigators associate shifts in the peritoneal response with disease mechanisms or treatment effects. The approach therefore supports time-dependent analysis of host responses instead of limiting interpretation to one experimental endpoint.
A typical workflow introduces sterile fluid into the peritoneal cavity, recovers it by lavage, and then prepares the sample for analysis. Depending on the research question, investigators may examine the recovered material by microscopy, count cells, perform immunophenotyping, culture infectious agents, or apply molecular assays. These measurements characterize local immune and infectious features.
The method is useful when the peritoneal cavity contains too little material for effective direct aspiration. Lavage provides a way to recover a sample that includes resident leukocytes and soluble factors from the localized site. In immunology and infection studies, this supports analysis of peritonitis, host-pathogen interactions, and treatment-related changes in the local response.
This approach can be used to investigate innate immune responses, characterize peritonitis, assess host-pathogen interactions, and evaluate treatment effects. Microscopy and cell counting describe cellular changes, immunophenotyping identifies immune-cell patterns, and culture or molecular assays examine infectious agents. Together, these outcomes connect local peritoneal events with underlying disease mechanisms.