Mir-155-5p
Mir-155-5p - Related Videos
09:58
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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Cited by 2 •
2017
This manuscript describes the efficient, non-viral delivery of miR to endothelial cells by a PEI/MNP vector and their magnetization. Thus, in addition to genetic modification, this approach allows for magnetic cell guidance and MRI detectability. The technique can be used to improve the characteristics of therapeutic cell products.
07:45
Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy
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Cited by 1 •
2025
This article describes a protocol for the collection and detection of miR-15a from tears as a new diagnostic tool for diabetic retinopathy.
07:37
Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
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Cited by 10 •
2016
A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.
10:10
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Cited by 24 •
2017
This protocol describes a robust, reproducible and simple method of isolation and culture of myoblast progenitor cells from the skeletal muscle of adult and aged people. The muscles used here include foot and leg muscles. This approach enables the isolation of an enriched population of primary myoblasts for functional studies.
11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Cited by 7 •
2017
We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.
FAQs
Once mature, miR-155-5p recognizes complementary sequences in target messenger RNAs. This interaction can promote degradation of those transcripts or reduce their translation, so the same regulatory molecule can alter how much protein is produced without changing the underlying gene sequence. In immune studies, this provides a mechanistic link between microRNA abundance and changes in cellular function.
Changes in miR-155-5p can shift the expression of multiple target messenger RNAs, making its effects broader than a single protein measurement. In activated immune cells, those shifts may influence cytokine production and lymphocyte function. The direction and magnitude of an observed response therefore depend on how miR-155-5p abundance changes and which target transcripts are affected.
Transcription and maturation are important because the functional species is the mature, approximately 22-nucleotide RNA rather than the initial transcript. Studying these stages helps distinguish altered production or processing from later changes in target regulation. That distinction matters when interpreting why immune or infection-related samples show different miR-155-5p levels.
Measurement of miR-155-5p abundance can be paired with observations of cytokine production, lymphocyte function, or pathogen-related responses. This combined view helps researchers ask whether an expression change coincides with an immune outcome, rather than treating the microRNA level as an isolated result. Such analyses may clarify relationships between regulatory RNA activity and host defense.
Manipulating miR-155-5p allows researchers to test whether changing its abundance alters immune or inflammatory behavior. Observing resulting changes in cytokine production, lymphocyte function, or pathogen-related responses can help evaluate a possible regulatory contribution. This approach supports functional investigation of how the microRNA participates in immune responses rather than only documenting its abundance.
In infectious and inflammatory disease research, miR-155-5p is relevant because altered abundance may reflect or influence host responses to pathogens. Researchers may investigate it as a potential biomarker or as part of a targeted strategy, depending on whether the goal is to track disease-related immune activity or explore regulation of the response.