Porphyromonas Gingivalis
Porphyromonas Gingivalis - Related Videos
02:37
Assessing Internalized Porphyromonas gingivalis in Host Endothelial Cells
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2025
In this video, we demonstrate an assay to assess the survival of Porphyromonas gingivalis inside human endothelial cells. The measure of survival and growth of internalized Porphyromonas gingivalis is evaluated by the number of colonies on the blood agar plate.
12:16
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
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Cited by 17 •
2015
This article presents two protocols: one to measure anaerobic bacteria that can successfully invade and survive within the host, and the other to visualize anaerobic bacteria interacting with host cells. This study can be applied to any cultivable anaerobe and any eukaryotic cell type.
05:28
A Bacterial Infection Assay for Studying Anaerobic Bacteria-Host Cell Interactions
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2025
This video demonstrates a technique for assessing the ability of Porphyromonas gingivalis, an anaerobic pathogenic bacterium, to adhere to and invade human umbilical vein endothelial cells (HUVECs). After infecting the host cells with the bacteria, the host cells are lysed to release both adhered and internalized bacteria. When the lysate is plated in an appropriate medium, the presence of colonies indicates successful bacteria-host cell interactions.
04:19
Visualization of Internalized Anaerobic Bacteria within Host Endothelial Cells
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2025
In this video, we demonstrate a procedure to visualize internalized Porphyromonas gingivalis, an anaerobic pathogenic bacterium, within human endothelial cells using confocal microscopy. The bacteria are stained with a fluorescent dye while the host endothelial cell actin cytoskeleton is labeled with a different fluorescent dye. The internalized Porphyromonas gingivalis inside endothelial cells are visualized using confocal microscopy.
09:52
A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites
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Cited by 15 •
2014
Animal models have proven to be invaluable tools in defining host and pathogen specific mechanisms that contribute to the development of chronic inflammation. Here we describe a mouse model of oral infection with the human pathogen Porphyromonas gingivalis and detail methodologies to assess the progression of inflammation at local and systemic sites.
FAQs
Gingipain proteases contribute to persistence by helping Porphyromonas gingivalis acquire nutrients and interfere with host defenses. Their activity can disrupt complement and inflammatory signaling, weakening or redirecting immune responses at the infection site. Studying these proteases helps explain how a bacterium remains established in subgingival environments while contributing to prolonged, tissue-damaging inflammation.
Fimbriae primarily support attachment to tissues within subgingival biofilms, helping maintain the organism’s position in the gingival environment. Lipopolysaccharide contributes to interactions with host immune pathways and can alter inflammatory signaling. Considering these factors separately allows investigators to distinguish mechanisms of colonization from mechanisms that influence immune dysregulation and chronic disease.
The subgingival biofilm provides a relevant setting for examining how Porphyromonas gingivalis interacts with tissues and neighboring microbes. Because periodontal disease involves polymicrobial communities, studying this environment helps researchers move beyond a single-microbe view of infection. It also supports investigation of how community interactions influence immune responses, persistence, and periodontal tissue damage.
Porphyromonas gingivalis serves as a model for examining how microbial factors interfere with host immunity. Researchers can focus on its proteases, fimbriae, and lipopolysaccharide to assess effects on complement, inflammatory signaling, and broader immune responses. These studies clarify how persistent microbial stimulation can produce immune dysregulation rather than an effective response that rapidly resolves infection.
Studies can examine how microbial persistence, immune disruption, and chronic inflammation relate to periodontal tissue destruction. The organism also provides a framework for investigating host-microbe interactions within polymicrobial communities. Findings may guide research into diagnostic approaches, antimicrobial interventions, and preventive strategies aimed at limiting disease-associated microbial activity or its effects on host tissues.
Its relevance extends beyond identifying a periodontal pathogen. Porphyromonas gingivalis allows investigators to connect specific virulence factors with altered complement activity, inflammatory signaling, and immune responses in a tissue-associated setting. This makes it useful for studying how infection can persist while promoting chronic inflammation, and for evaluating approaches to diagnosis, treatment, and prevention.