Recombination Efficiency
Recombination Efficiency - Related Videos
13:47
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
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Cited by 8 •
2012
Here we describe a protocol based only on cell infection, which improves the efficiency of recombinant parvovirus production by more than 100 fold in comparison to other protocols in use. This protocol relies on the use of a novel adenovirus 5-based helper containing the parvovirus VP transcription unit (Ad-VP).
07:50
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
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Cited by 200 •
2013
Plants offer a novel system for the production of pharmaceutical proteins on a commercial scale that is more scalable, cost-efficient and safe than current expression paradigms. In this study, we report a simple and convenient, yet scalable approach to introduce target-gene containing Agrobacterium tumefaciens into plants for protein transient expression.
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 9 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
13:52
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
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Cited by 3 •
2014
Kindlins are fundamental to cell adhesion through integrins but studies of them have been hampered by the difficulty encountered in expressing them recombinantly in bacterial hosts. We describe here methods for their efficient production in baculovirus-infected insect cells.
19:02
High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
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Cited by 29 •
2011
In this article, we describe the identification of the adeno-associated virus serotype 3 (AAV3) as the most efficient vector for targeting human liver cancer cells.
FAQs
During V(D)J recombination, RAG1 and RAG2 recognize recombination signal sequences and initiate the DNA cleavage required for segment joining. Recombination efficiency therefore depends on successful progression from signal recognition to repair, rather than cleavage alone. Studying this sequence helps connect molecular events with the production of correctly assembled antigen-receptor genes.
High efficiency supports the assembly of a broader set of antibody and T-cell receptor genes, increasing the potential diversity of antigen receptors. That diversity is biologically important because developing lymphocytes need functional receptors to populate B- and T-cell compartments. Consequently, changes in joining performance can affect both receptor variety and immune-cell development.
After RAG1 and RAG2 create DNA breaks, nonhomologous end joining repairs the cleaved ends and completes the joining process. This repair stage is central to whether a recombination event produces a correctly assembled gene. Examining it separately from signal recognition can clarify why cleavage may not always translate into productive receptor formation.
Researchers can frame measurement around three outcomes: whether DNA segments joined correctly, how much product was generated, and how much diversity appeared among the resulting genetic products. These readouts distinguish a process that produces many joins from one that produces accurate, diverse, and potentially functional antigen-receptor genes, supporting comparisons across immune-development studies.
Manipulating recombination efficiency is especially relevant when investigating immune development, immunodeficiency, or susceptibility to infection. A change in joining performance can be examined for its relationship to antigen-receptor diversity and the formation of functional B- and T-cell populations. This makes efficiency a useful experimental variable for connecting gene assembly with immune outcomes.
In engineered immune-cell research, recombination efficiency provides a way to evaluate strategies intended to improve immune-cell responses. The key question is whether optimized gene assembly increases the production of useful antigen-receptor configurations while preserving correct joining. Such analyses connect molecular recombination behavior with the design of more effective engineered immune-cell populations.