Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

This experimental system uses agro infiltration to effectively deliver gene constructs to leaves for the production of high levels of recombinant protein. First, grow end hamana plants in a controlled environment for six weeks, three days before infiltration. Prepare the agrobacterium cultures which contain the target gene to be expressed in the plants on the day of infiltration.

Introduce the agrobacterium cultures into the plant tissue by either syringe or vacuum infiltration. Results like the intensity of green fluorescence of GFP in tobacco leaves under UV light demonstrate a robust expression of recombinant proteins in plants that rivals that of current mammalian bacterial and insect based expression systems. Agro infiltration is a effective way to introduce trans genes into plant cells, which lead to high level expression of recombinant proteins.