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Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
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JoVE Journal Biology
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

DOI:
10.3791/50521-v

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07:50 min

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July 23, 2013

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, , , , , ,
1The College of Technology and Innovation, Center for Infectious Disease and Vaccinology, The Biodesign Institute,Arizona State University

Chapters

  • 00:05Title
  • 01:31Plant Growth
  • 02:24Infiltration by Syringe
  • 03:44Infiltration by Vacuum
  • 04:51Representative Results
  • 06:47Conclusion

Summary

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Plants offer a novel system for the production of pharmaceutical proteins on a commercial scale that is more scalable, cost-efficient and safe than current expression paradigms. In this study, we report a simple and convenient, yet scalable approach to introduce target-gene containing Agrobacterium tumefaciens into plants for protein transient expression.

Tags

Keywords: AgroinfiltrationTransient ExpressionRecombinant ProteinsPlant-based ProductionGFPDsRedSyringe InfiltrationVacuum InfiltrationPharmaceutical ProteinsBiotechnology Education

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