Human Adipose Sampling
Human Adipose Sampling - Related Videos
07:24
Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy
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Cited by 4 •
2017
Videomicroscopy systems are used to examine functional properties of isolated adipose tissue arterioles in response to physiological and pharmacological stimuli. This technique can be used to examine microvascular phenotypes in different adipose tissue domains in obese humans.
08:59
Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique
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Cited by 5 •
2021
The manuscript and associated video demonstrate a percutaneous biopsy technique to obtain samples of subcutaneous adipose tissue from areas surrounding the umbilicus. This method is a low-risk and efficient way to investigate a range of parameters (e.g., gene or protein expression, enzyme activity, lipid content) within adipose tissue.
07:23
Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
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Cited by 136 •
2013
In 2001, researchers at UCLA described the isolation of a population of adult stem cells, termed Adipose-derived Stem Cells or ASCs, from adipose tissue. This article outlines the isolation of ASCs from lipoaspirates using a manual, enzymatic digestion protocol using collagenase.
09:20
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Cited by 47 •
2016
This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.
07:35
Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
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Cited by 16 •
2015
Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.
FAQs
Adipose tissue contains more than stored fat. Its cellular and structural components can be examined through adipocytes, stromal vascular cells, immune cells, extracellular matrix, nucleic acids, and lipids. Studying these components separately or together helps investigators connect tissue composition with obesity-related inflammation, insulin resistance, metabolic disease, and the biology of adipose-derived stem or stromal cells.
Sampling site, preservation, and processing influence how consistently specimens can be compared. Adipose tissue contains multiple cell populations and molecular materials, so inconsistent collection or handling may affect which analytes remain available for study. Standardized procedures strengthen reproducibility, making results more useful for biomarker studies, therapeutic research, and comparisons among clinical or research samples.
After collection, the specimen is preserved and processed according to the intended analysis. This workflow can support isolation of adipocytes, stromal vascular cells, immune cells, nucleic acids, or other analytes from the tissue. Separating these targets allows researchers to examine cellular, structural, and molecular features rather than treating adipose tissue as a single uniform material.
Clinical assessment focuses on characterizing tissue in relation to a person's health, while biomedical research uses specimens to investigate mechanisms, biomarkers, and potential therapies. The same collected material may support different analyses depending on the study goal. This distinction helps determine which components to preserve and which analytes or cell populations are most relevant.
The general workflow proceeds from controlled collection to preservation, processing, and analysis. Researchers first maintain the specimen in a form suitable for the planned investigation, then isolate selected cells, nucleic acids, lipids, extracellular matrix, or other analytes. Linking each analytical result to the sampling site and handling procedure improves interpretation and reproducibility.
In medicine, these specimens provide tissue-level evidence for studying obesity-related inflammation, insulin resistance, and metabolic disease. They also support investigations of adipose-derived stem or stromal cells, biomarker development, and therapeutic research. The resulting analyses can clarify how adipose tissue contributes to whole-body health and help connect local tissue characteristics with broader clinical questions.