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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

DOI:
10.3791/53886-v

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09:20 min

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March 31, 2016

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, , , , , ,
1Institute of Biotechnology,National Taiwan University, 2Department of Animal Science and Technology,National Taiwan University, 3Department of Surgery,National Taiwan University Hospital and College of Medicine

Chapters

  • 00:05Title
  • 01:10Pig Subcutaneous Adipose Tissue Dissection
  • 04:11Stromal-Vascular Fraction Primary Adipose-Derived Stem Cells (pADSC) Collection
  • 06:14Results: Representative pADSC Morphology, Phenotype and Differentiation
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.

Tags

Keywords: Adipose-derived Stem CellsMesenchymal AdipocytesOsteocytesChondrocytesObesity ResearchAdipocyte DifferentiationStem Cell TherapyMultipotencyTransdifferentiation

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